Genome-wide association study to identify genes and biological pathways related to resistance to bovine tuberculosis based on gene set enrichment analysis

Articles in Press

Document Type : Research Paper

Authors

Department of Animal Science, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran.

Abstract

The aim of the present study was to conduct a genome-wide association study (GWAS) to identify the genes and biological pathways related to resistance to bovine tuberculosis based on gene set enrichment analysis. In total, the information of 1355 cows (consisting 685 cases and 670 controls) from 178 herds were used, of which 592 cases and 559 controls (healthy cows) were passed different quality control steps. These animals were genotyped by using Illumina BovineHD 700k BeadChip for 727252 SNP markers. Genome-wide association study (GWAS) was investigated using mixed linear logistic models, and regions of the genome that were associated with bovine tuberculosis at the 0.05 level were used for identifying the candida genes and biological pathways associated with bovine tuberculosis based on gene set enrichment analysis. The results of this study finally led to the identification of the pathways that were significantly associated with bovine tuberculosis. Further investigation of these pathways showed that most of them are related to the immune system, stress and disease resistance. Also, study of function of the genes that were located in these pathways revealed that some of these genes such as HSPD1, BCL6, KCNMA1, SPP1, CD24, CD80, MYO1B, CCL20, SLC11A1, LCP2 and SLC2A2, have also been confirmed to be directly or indirectly associated with disease resistance and bovine tuberculosis in previous studies.

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Main Subjects

Iranian Journal of animal Science

Articles in Press, Accepted Manuscript
Available Online from 15 April 2025

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History

  • Receive Date: 21 December 2024
  • Revise Date: 30 January 2025
  • Accept Date: 16 February 2025

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