Evaluation different levels of soy lecithin on in vitro preservation of turkey sperm

Document Type : Research Paper

Authors

1 Department of Animal Science, Faculty of Agriculture, University of Tehran. Karaj. Iran

2 Department of Animal Science, Faculty of Agriculture, Urmia University

Abstract

This study was conducted in order to investigate different levels of soy lecithin in semen diluent on the qualitative characteristics of turkey sperm in in vitro storage conditions. Semen samples were collected from five heavy turkeys of the French bronze breed at the age of 36 weeks. After initial evaluations (more than 70% dilution), they were mixed together and divided into four equal parts, and each part was diluted with one of the following diluents: 1) Control medium (10% egg yolk), 2) medium containing 1% lecithin, 3) medium containing 2% lecithin, 4) medium containing 3% lecithin. Different sperm parameters including total and progressive motility, integrity and plasma membrane activity were evaluated 3 hours after storage at 4°C. According to the obtained results, egg yolk-based medium and medium containing 2% lecithin showed the highest percentage of total motility (77.5%). Egg yolk-based medium showed the highest percentage of progressive motility (61.5%). However, there was no significant difference with other treatments. The results of the membrane activity showed that the treatment containing 2% lecithin showed the highest percentage with a significant difference compared to other treatments (85%) (P<0.05). Also, the evaluation of sperm viability and morphology was not affected by the treatments. Therefore, it seems that the egg yolk-based medium and the medium containing 2% soy lecithin reduce the negative effects of in vitro storage of turkey sperm at 4°C.

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Articles in Press, Accepted Manuscript
Available Online from 15 April 2025
  • Receive Date: 29 October 2024
  • Revise Date: 01 January 2025
  • Accept Date: 24 February 2025