Effect of different concentrations of ether extract of propolis on the sperm characteristics of Zel ram

Articles in Press

Document Type : Research Paper

Authors

1 Department of Animal Sciences, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

2 Department of Animal science, Faculty of Agriculture, University of Guilan, Rasht,, Iran

Abstract

Abstract
In the present study, the effect of ether extract of propolis on the semen characteristics of Zel's ram under short and long-term preservation was investigated. The experimental groups comprised different concentrations of ether extract (0, 0.25, 0.5, and 1 mg/mL) and dimethyl sulfoxide (DMSO). The samples were evaluated at 0, 24, 48, and 72 hours after storage at 4 °C and following the freeze-thawing process. During the cold storage, sperm quality traits motility, viability, membrane integrity, mitochondrial activity, malondialdehyde concentration (MDA), and morphology increased significantly (P<0.05) at concentrations of 0.25 and 0.5 mg/mL ether extract in comparison with the control group. Our results revealed that the total motility, viability, and membrane integrity in the concentration of 0.5 mg/mL ether extract were higher than the control group (P<0.05) at 4 °C. Also, motility, viability and malondialdehyde concentration (MDA) were higher in concentrations of 0.25 mg/mL ether extract than in the control group in the freeze-thawing process. Mitochondrial activity and morphology of spermatozoid at 0.25 and 0.5 mg/mL ether extract were higher than the control group (P<0.05) in storage at 4 °C and freeze-thawing process. Results showed adding ether extract at the concentrations of 0.25 and 0.5 mg/mL to the semen extender improved the quality of spermatozoid in the Zel breed sheep.

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Iranian Journal of animal Science

Articles in Press, Accepted Manuscript
Available Online from 03 February 2025

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History

  • Receive Date: 03 December 2024
  • Revise Date: 03 January 2025
  • Accept Date: 18 January 2025

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