Isolation and biological evaluation of lactic acid bacteria from bee pollen and bee bread

Articles in Press

Document Type : Research Paper

Authors

1 Department of Animal Sciences, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran.

2 Department of Honeybee, Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

The aim of the present research was to isolate lactic acid-producing bacteria from pollen or bee bread. For this purpose, bacterial strains were isolated from various samples of pollen and bee bread using MRS culture medium. The characteristics of the isolates were assessed through Gram staining, catalase activity tests, fermentation of specific sugars, growth curve analysis, pH reduction in the culture medium, and gas production. The results from the growth curve analysis of the isolated bacteria demonstrated significant differences in their growth rates under identical culture conditions, indicating varying capacities for pollen fermentation. Specifically, bacteria MB1, MB2, MB4, and MP1 exhibited robust growth during the initial 48 hours. In contrast, bacteria MB3 and MP2 remained in the lag phase throughout this period; however, MP2 and MB4 subsequently experienced a marked increase in growth rate, with highest optical density (OD). Following incubation of the pollen-containing culture medium, bacterial activity in fermenting pollen was evaluated based on lactic acid production. All isolated bacteria were confirmed to be Gram-positive and catalase-negative, demonstrating the ability to ferment certain simple sugars. The isolated strains exhibited varying efficiencies in both growth rates and lactic acid production, suggesting differences in their capacity to utilize the nutrients found in pollen. However, no significant differences were observed among the bacterial strains concerning gas production.

Keywords

Iranian Journal of animal Science

Articles in Press, Accepted Manuscript
Available Online from 03 February 2025

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History

  • Receive Date: 30 November 2024
  • Revise Date: 13 January 2025
  • Accept Date: 20 January 2025

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