Seyed Nader Albooshoke; Mohammad Reza Bakhtiarizadeh; Mojtaba Tahmoorespur
Abstract
This experiment was conducted to investigate and identify the isoforms related to the structure of muscular proteins between Isfahani native and Ross commercial chickens with different growth rates. We extracted total RNA from breast muscle samples of two groups at end of 4 weeks of age. After paired-end ...
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This experiment was conducted to investigate and identify the isoforms related to the structure of muscular proteins between Isfahani native and Ross commercial chickens with different growth rates. We extracted total RNA from breast muscle samples of two groups at end of 4 weeks of age. After paired-end sequencing of samples using the Illumina Hiseq 2000 platform, Hisat2 was applied to align clean reads to chicken reference genome. Then, Cufflinks package was used to assemble transcripts and identify significantly differentially expressed genes. The statistical comparison of the isoforms between two groups revealed 259 isoforms with significant difference in expression, of which 161 isoforms were up-regulated and 98 isoforms were downregulated in commercial chickens. Among the commercial chicken isoforms, four genes (ACTC1, ATF3, CYR61 and FABP4) were upregulated with two different isoforms. In addition, in commercial chicks, the frequency of isoforms associated with slow contraction fibers was greater than that of rapid contraction fibers. Functional study showed that the isoforms in commercial chickens were more related to cell proliferation and differentiation, hypertrophy growth and biosynthesis of muscle proteins, whereas in native chickens, mainly they were associated to immune processes, carriers of ions and metals, binding to metals, DNA and RNA, and factors contributing to degradation of muscle proteins. The results of this study showed that such changes may have been able to strengthen the ability to maintain and overcome the severe environmental and nutritional conditions during the developmental period of the chicken by reducing the level of requirements and enhancing immunity and adaptability in native chicken.
Zohre Mozduri; Mohammad Reza Bakhtiarizadeh; Abdolreza Salehi
Abstract
Negative energy balance (NEB) occurs inhigh-producing dairy cows in first few weeks after parturition, that energy demand for maintenance and milk production exceeds the dietary energy intake. NEB has a considerable economic importance due to negative effect on health and fertility in dairy herds, therefore, ...
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Negative energy balance (NEB) occurs inhigh-producing dairy cows in first few weeks after parturition, that energy demand for maintenance and milk production exceeds the dietary energy intake. NEB has a considerable economic importance due to negative effect on health and fertility in dairy herds, therefore, the identification of its effective regulatory mechanism is important. miRNAs are one of these effective regulatory factors in NEB. Despite of the importance of NEB, the regulatory mechanisms related to miRNAs has not been well documented. In this study miRNA-seq data from liver tissue of eight Holstein dairy cows were analyzed to identify new miRNAs and isomirs. All data have been achieved from GEO in NCBI database. A total of 291 new miRNAs with homologous gene in other species were identified. Moreover, 164 new miRNAs without homologous were identified. Investigation of target genes of these miRNAs lead to identify biological paths related to NEB. Also 466 new isomiR and 95 new miRNA* were detected for the first time in cow genome. The results of the current study provide new information for better understanding of the regulatory roles of miRNAs in NEB.
mohammadreza bakhtiarizade; mohammad moradi shahre babak; hossein moradi; mahmood vatankhah
Abstract
The relationship between live body weight, body length, girth circumference, animal hight, upper, middle as well as lower width of fat-tail, fat-tail length, fat-tail gap length, fat-tail depth and fat-tail circumference along with fat-tail weight were determined using records of 731 Loribakhtiari sheep. ...
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The relationship between live body weight, body length, girth circumference, animal hight, upper, middle as well as lower width of fat-tail, fat-tail length, fat-tail gap length, fat-tail depth and fat-tail circumference along with fat-tail weight were determined using records of 731 Loribakhtiari sheep. Principal Component and Least Square Analyses were applied to solve the collinearity instability. Collinearity problems as portrayed by variance inflation factors above 5 or 10 were evident in some of independent variables. Results showed that the problem of collinearity in relation with fat-tail weight of 11 independent variables could be solved by using Principal Component Analysis method. Fat-tail gap length, fat-tail depth, and fat-tail circumference vs. girth circumference, and fat-tail length respectively represented the highest and the lowest coefficients regarding the estimation of fat-tail weight.
MohammadReza BakhtiariZadeh; Mohamad Moradi ShahrBabak
Volume 41, Issue 1 , May 2010
Abstract
The objective of the present study was to estimate the lactation curve parameters through incomplete gamma function and to genetically evaluate the relationship of these parameters and udder traits in Holstein population of Iran. Two data sets' including 117583 [(for lactation curve parameters) and 7351 ...
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The objective of the present study was to estimate the lactation curve parameters through incomplete gamma function and to genetically evaluate the relationship of these parameters and udder traits in Holstein population of Iran. Two data sets' including 117583 [(for lactation curve parameters) and 7351 (for determining genetic and phenotypic relationship between lactation curve parameters and udder type traits)], first lactation records on cows from 19303 and 1148 herd-year-season groups were respectively employed. Heritability estimates varied from 0.02 (a and b) to 0.22 (peak) and for udder type traits ranged from 0.11 (FU) to 0.21 (RTP).The genetic correlations among lactation curve parameters ranged from 0.99 (TTP and PERS) to -0.64 (a and b) and among udder type traits and lactation curve parameters ranged from 0.54 (RTP and a, RUW and PEAK) to -0.54 (RTP and b). Results suggested that the application of RUW for indirect selection of lactation curve parameters could be useful for genetic improvement of persistency in milk yield. With regard to the present study, cows with more udder width had a more M305, PEAK as well as PERS.
MohammadReza BakhtiariZadeh; Mohammad Moradi ShahreBabak; Abbas Pakdel; Ahmad Moghimi
Volume 40, Issue 4 , March 2010
Abstract
The objective of the present study was to estimate the relationship between genetic parameters for 13 linear type traits, and milk yield as well as open day in Holstein cattle of Iran. Three data sets of: 16502 (type traits), 16557 (milk yield) and 11631 (open days) records related to the first calving ...
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The objective of the present study was to estimate the relationship between genetic parameters for 13 linear type traits, and milk yield as well as open day in Holstein cattle of Iran. Three data sets of: 16502 (type traits), 16557 (milk yield) and 11631 (open days) records related to the first calving of the cows, and respectively taken from 1500, 1566, and 2000 herd-year-season groups, were taken into account. SAS software and Proc GLM were employed to fit the model. Genetic parameters were estimated using Restricted Maximum Likelihood along with an animal model and ASREML software. Genetic correlation among type traits and milk production ranged from 0.34 (rear udder width) to -0.31 (udder depth), and among type traits and fertility, it ranged from 0.62 (rear udder width) to -0.37 (udder depth). Most of the genetic correlations between milk production and fertility indicate that more production in cows was accompanied by a lower fertility, while cows with a lower production level benefitted from a more fertility rate.
