Iranian Journal of animal Science

Iranian Journal of animal Science

Effect of diluted semen temperature and sperm concentration on sperm penetration ‎rate, fertility and hatchability of aged broiler breeder hens

Document Type : Research Paper

Authors
Hossein Sharideh 1, 2
Mojtaba Zaghari 3
Seyed Jafar Mirbehbahani 1
1 Researcher of ETKA Organization ‎
2 Ph.D. Graduate in Animal Physiology, Department of Animal Science, College of Agriculture and Natural ‎Resources, University of Tehran, Karaj, Iran
3 Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijas.2020.307142.653792
Abstract
The current study aimed to optimize artificial insemination in aged broiler breeder hens in two experiments. In the first experiment, the effect of (two) different diluted semen temperatures (5 and 25 °C) of Hubbard rooster (40 roosters, 58 weeks of age) on fertility, hatchability and sperm penetration (SP) rate in the perivitelline layer of Hubbard hen (180 hens) were investigated. In the second experiment, three (different) sperm concentrations (100 (C100), 200 (C200), and 400 (C400) million sperm in 0.25 mL per hen) of Hubbard roosters (40 roosters, 62 weeks of age) on fertility, hatchability and SP rate of Hubbard broiler breeder hens (270 hens) were explored. In the first experiment, the results showed that the temperature of 5 °C of diluted semen compared to the 25 °C, increased percentage of hatchability of set eggs, hatchability of fertile eggs, and SP and decreased early embryonic mortality. The results of the second experiment showed the highest percentage of fertility and SP rate were observed at treatment C400. Also, in this experiment that highest percentage of hatchability of set eggs and hatchability of fertile eggs and lowest early embryonic mortality were observed at treatment C400. Return on investment (ROI) of the treatments C200 and C400 was approximately 2.9 and 1.4, respectively. In overall, the results of this study showed that (in attention to ROI and hatchability) to optimize artificial insemination of aged broiler breeder hens we can use a sperm concentration of 200 to 400 million in 0.25 mL per hens at 5 °C.
Keywords
Fertility
Extender temperature
Rooster
Hatchability
Sperm penetration‎
  1. Bakst, M. R. & Dymond, J. S. Artificial insemination in poultry. In: Lemma A., editor. Success in artificial insemination quality of semen and diagnostics employed: InTech; 2013. p. 176-89.
  2. Bramwell, R. K., Marks, H. L. & Howarth, B. (1995). Quantitative determination of spermatozoa penetration of the perivitelline layer of the hen's ovum as assessed on oviposited eggs. Poultry Science, 74, 1875-83.
  3. Burrows, W. H. & Quinn, J. P. (1937). The Collection of Spermatozoa from the Domestic Fowl and Turkey. Poultry Science, 16, 19-24.
  4. Christensen, V. L., Fairchild, B. D. & Ort, D. T. (2005). The Relationship Between Sperm Hydrolysis of the Perivitelline Layer and Embryonic Livability1. Journal of Applied Poultry Research, 14, 60-8.
  5. Das, S. C., Isobe, N., Nishibori, M. & Yoshimura, Y. (2006). Expression of transforming growth factor-β isoforms and their receptors in utero-vaginal junction of hen oviduct in presence or absence of resident sperm with reference to sperm storage. Reproduction (Cambridge, England), 132, 781-90.
  6. Donoghue, A. M. (1996). The effect of twenty-four hour in vitro storage on sperm hydrolysis through the perivitelline layer of ovipositioned turkey eggs. Poultry Science, 75, 1035-8.
  7. Eslick, M. L. & McDaniel, G. R. (1992). Interrelationships Between Fertility and Hatchability of Eggs from Broiler Breeder Hens. Journal of Applied Poultry Research, 1, 156-9.
  8. Hocking, P. M. (1990). The relationships between dietary crude protein, body weight, and fertility in naturally mated broiler breeder males. British Poultry Science, 31, 743-57.
  9. Hocking, P. M. & Bernard, R. (2000). Effects of the age of male and female broiler breeders on sexual behaviour, fertility and hatchability of eggs. British Poultry Science, 41, 370-6.
  10. Long, J. A. & Conn, T. L. (2012). Use of phosphatidylcholine to improve the function of turkey semen stored at 4 degrees C for 24 hours. Poultry Science, 91, 1990-6.
  11. Long, J. A. & Kramer, M. (2003). Effect of vitamin E on lipid peroxidation and fertility after artificial insemination with liquid-stored turkey semen. Poultry Science, 82, 1802-7.
  12. Meyer, G. B., Props, C. F., Leighton, A. T., Jr., Van Krey, H. P. & Potter, L. M. (1980). Influence of dietary protein during the pre-breeder period on subsequent reproductive performance of large white turkeys. 3. The effects of semen volume and frequency of insemination on fertility and hatchability. Poultry Science, 59, 363-8.
  13. Moghbeli, M., Kohram, H., Zare-Shahaneh, A., Zhandi, M., Sharideh, H. & Sharafi, M. (2016). Effect of sperm concentration on characteristics and fertilization capacity of rooster sperm frozen in the presence of the antioxidants catalase and vitamin E. Theriogenology, 86, 1393-8.
  14. Sarabia Fragoso, J., Pizarro Diaz, M., Abad Moreno, J. C., Casanovas Infesta, P., Rodriguez-Bertos, A. & Barger, K. (2013). Relationships between fertility and some parameters in male broiler breeders (body and testicular weight, histology and immunohistochemistry of testes, spermatogenesis and hormonal levels). Reproduction in Domestic Animals, 48, 345-52.
  15. SAS Institute. (2009). SAS/STAT® User’s Guide, Release 8.02 ed. Statistical Analysis Systems Institute Inc., Cary, NC, USA.
  16. Schulze, M., Nitsche-Melkus, E., Hensel, B., Jung, M. & Jakop, U. (2020). Antibiotics and their alternatives in Artificial Breeding in livestock. Animal Reproduction Science. 106284, from https://doi.org/10.1016/j.anireprosci.2020.106284.
  17. Sexton, T. J. (1977). A new poultry semen extender. 1. Effects of extension on the fertility of chicken semen. Poultry Science, 56, 1443-6.
  18. Sexton, T. J. (1988). Comparison of Commercial Diluents for Holding Turkey Semen 24 Hours at 5 C. Poultry Science, 67, 131-4.
  19. Sharideh, H., Esmaeile Neia, L., Zaghari, M., Zhandi, M., Akhlaghi, A. & Lotfi, L. (2016 a). Effect of feeding guanidinoacetic acid and L-arginine on the fertility rate and sperm penetration in the perivitelline layer of aged broiler breeder hens. Journal of Animal Physiology and Animal Nutrition, 100, 316-22.
  20. Sharideh, H., Zhandi, M., Zeinoaldini, S., Zaghari, M. & Sadeghi, M. (2019). The effect of coenzyme Q10 on rooster semen preservation in cooling condition. Theriogenology, 129, 103-109.
  21. Sharideh, H., Zhandi, M., Zaghari, M. & Akhlaghi, A. (2016 b). Dietary zinc oxide and Escherichia Coli-derived 6-phytase improve the fertility rate in old broiler breeder hens with no effect on immunity in their progeny hatchlings. Journal of Agricultural Science and Technology, 18, 327-336.
  22. Sharideh, H., Zhandi, M., Zeinoaldini, S., Zaghari, M., Sadeghi, M., Akhlaghi, A. & Peebles, E. D. (2020). Beneficial effects of dietary coenzyme Q10 on the productive and reproductive variables of broiler breeder hens. Animal Reproduction Science, 213, 106256.
  23. Vasicek, J., Kuzelova, L., Kulikova, B. & Chrenek, P. (2015) Effect of diluent and storage timeon sperm characteristics of rooster insemination doses. Avian Biology Research, 8, 41-46.
  24. Vizcarra, J.A., Kirby, J.D., Kreider & D.L. (2010). Testis development and gonadotropin secretion in broiler breeder males. Poultry Science, 89, 328-334.
  25. Waberski, D., Luther, A.-M., Grünther, B., Jäkel, H., Henning, H., Vogel, C., Peralta, W. & Weitze, K. F. (2019). Sperm function in vitro and fertility after antibiotic-free, hypothermic storage of liquid preserved boar semen. Scientific Reports, 9, 14748.
  26. Zaghari, M., Derakhshani-Diba, M., Moravej, H. & Zahroojian, N. (2017). Estimation of metabolizable energy equivalency of Bacillus subtilis spore in male broiler chickens. Journal of Livestock Science and Technologies, 5, 9-18.
  27. Zamiri, M. J., Hashemi, M. R. &  Boostani, A. (2005). An evaluation of several semen diluents for storageof fars native chicken sperm at 4-5 and 19-24 °C. Iranian Journal of Agriculture Science, 36, 603-612. (In Farsi).
Iranian Journal of animal Science
Volume 52, Issue 2
Summer 2021
Pages 133-140

  • Receive Date 02 August 2020
  • Revise Date 08 October 2020
  • Accept Date 13 October 2020