Iranian Journal of animal Science

Iranian Journal of animal Science

Improvement of testis histological parameters and relative expression of StAR gene in Chrysin-fed roosters

Document Type : Research Paper

Authors
Ali Sami Amin Altawash 1
Ahmad Zareh Shahneh 2
Hossein Moravej 2
Mahdi Ansari 1
Hamid Deldar 3
1 Former Ph.D. Student, Animal Science Department, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Animal Science Department, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Department of Animal Science, College of Animal Science and Fisheries, Sari Agricultural Science Natural Resources University, Sari, Iran
10.22059/ijas.2018.240313.653554
Abstract
Chrysin was orally administered to evaluate testicular histology and relative StAR expression of Ross 308 roosters in the current study. Twenty roosters were randomly divided into four groups and were subjected to the basal diet supplemented with different levels of Chrysin including 0 (Ch0), 25 (Ch25), 50 (Ch50) or 75 (Ch75) mg/bird/day for 12 successive weeks. At the end of trial, all birds were killed and two samples were collected from the same testicle one of which was processed for histology, whereas another was snap-frozen with liquid nitrogen to assess relative gene expression. According to the results, seminiferous tubule and epithelium diameters (p<0.05) and spermatogonial numbers (p<0.01) of both CH75 and Ch50 groups were significantly improved compared to control group. However, Leydig cell numbers and blood vessels were not significantly affected. Birds fed 75 mg of Chrysin per day had significantly higher StARtranscript level compared to other groups (p<0.05). In conclusion, oral administration of Chrysin to roosters could improve testicular histology and steroidogenic parameters in the current study.
Keywords
Chrysin
gene
histology
rooster
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Iranian Journal of animal Science
Volume 50, Issue 3
Autumn 2019
Pages 207-215

  • Receive Date 28 August 2017
  • Revise Date 09 January 2018
  • Accept Date 09 January 2018