اثر یک خوراک غنی از ترکیبات فنلی بر بیان ژن‌های آمیلاز و Actin-β در بافت‌های کبد و پانکراس جوجه‌های گوشتی

نوع مقاله : مقاله پژوهشی


1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه یاسوج

2 دانشیار، دانشکده کشاورزی، دانشگاه یاسوج

3 استادیار، دانشکده کشاورزی، دانشگاه یاسوج


این پژوهش به‌منظور بررسی میزان بیان ژن آمیلاز و Actin-β در بافت‌های کبد و پانکراس جوجه‌های گوشتی تغذیه شده با سطوح مختلف میوه‌ بلوط انجام شد. تعداد 264 قطعه جوجه گوشتی با جیره شاهد (بدون میوه بلوط) و جیره‌های حاوی 15 و 20 درصد میوه بلوط تغذیه شدند. در پایان دوره‌ آغازین و پایانی، از هر تیمار آزمایشی، 6 قطعه جوجه به‌طور تصادفی انتخاب و نمونه‌گیری از بافت پانکراس و کبد انجام شد. جهت آنالیز بیان ژن از نرم‌افزارهای ­REST وSAS  استفاده شد. از نرم‌افزار 2004Bestkeeper نیز به‌منظور بررسی پایداری بیان ژن Actin-β استفاده شد. نتایج نشان داد که ژن Actin-β تحت تأثیر عوامل جنس، سن و تیمارهای آزمایشی قرار نگرفت، بنابراین از آن به‌منظور نرمال ‌‌سازی داده‌های بیان ژن استفاده شد. در سن 21 روزگی، سطح mRNA ژن آمیلاز پانکراس در جوجه‌های گوشتی تیمار 15 درصد نسبت به گروه شاهد، به‌طور معنی‌داری بالاتر بود (05/0P<). در سن 42 روزگی، اختلاف معنی‌داری از نظر میزان بیان ژن آمیلاز کبد و پانکراس مشاهده نشد (05/0P>) همچنین، تغذیه جوجه‌ها با جیره‌ حاوی 20 درصد میوه‌ بلوط، موجب افزایش وزن نسبی پانکراس شد (05/0P<). به‌طور کلی، نتایج این بررسی نشان داد بیان ژن آمیلاز پانکراس در گروه تغذیه شده با جیره‌ حاوی 15 درصد میوه‌ بلوط در سن 21 روزگی افزایش معنی‌داری را نشان داد. در سایر موارد، بیان ژن‌ها تحت تأثیر تیمارهای آزمایشی قرار نگرفتند. 


عنوان مقاله [English]

Effect of a Phenolic compounds-rich feedstuff on expression of amylase and β-Actin genes in liver and pancreas tissues of broilers

نویسندگان [English]

  • Asma Moradalipour 1
  • Mostafa Muhaghegh-Dolatabady 2
  • Mohammad Houshmand 2
  • Mousa Zarrin 3
1 Former M.Sc. Student, Faculty of Agriculture Science, University of Yasouj, Yasouj, Iran
2 Assocaite Professor, Faculty of Agriculture Science, University of Yasouj, Yasouj, Iran
3 Assistnat Professor, Faculty of Agriculture Science, University of Yasouj, Yasouj, Iran
چکیده [English]

This study was conducted to determine the amylase gene expression in liver and pancreas tissues of broiler chicks fed with two levels of oak acorn(OA) fruit. A total of 264 broiler chicks were fed a control diet (without oak) and diets containing 15 and 20% OA. At the end of starter and finisher period, 6 birds from each treatment were randomly selected and Tissue samples were taken from their pancreas and liver. REST and SAS software were used to analyze gene expression. The 2004 Bestkeeper software was also used to determine the sustainability of β-Actin gene expression. Results showed that β-Actin gene was not affected by sex, age and experimental treatments. Thus, it was used to normalize the gene expression data. At 21d of age, the level of mRNA of the pancreatic amylase gene was significantly higher in broilers fed with 15% OA compared to the control group (p<0.05). On d 42, significant differences in expression of liver and pancreas amylase gene were not observed (p>0.05). Also, feeding birds with diet containing 20% OA increased the relative weight of pancreas (p<0.05). In conclusion, the results showed that amylase gene expression in birds fed diets containing 15% OA significantly increased at 21d of age. In other cases, genes expression was not influenced by experimental treatments.

کلیدواژه‌ها [English]

  • age
  • Amylase
  • β-actin
  • mRNA
  • Oak acorn
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