Iranian Journal of animal Science

Iranian Journal of animal Science

Evaluation of genes expression affecting mammary gland growth and development in cows fed fish oil

Document Type : Research Paper

Authors
Hoda Javaheri Barfourooshi 1, 2
Armin Towhidi 3
Hassan Sadeghipanah 2
Mahdi Zhandi 4
Saeed Zeinoaldini 4
Mohammad Hossein Banabazi 2
1 Former Ph. D. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Assistant Professor, Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3 Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
4 Associate Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijas.2018.242585.653562
Abstract
This study was conducted to evaluate the effects of feeding fish oil compared to palm oil on the expression of genes affecting the mammary gland growth and development in Holstein cows. Ten cows with at least one parturition were randomly assigned to fish oil (FO) or palm oil (PO) groups, from 42 days before expected parturition date up to 63 days of lactation. Blood samples were taken at 21 days intervals and mammary tissue sampling were taken in two-month intervals (seven and 63 days after parturition). There was no significant difference in serum estradiol and progesterone concentration between two groups. The result of gene expression analysis showed that the relative expression of IGF-I gene in the fish oil group decreased (P<0.05) and the relative expression of TNF-α gene in this group tended to decrease (P=0.06). Expression of Bcl-2 in fish oil group tended to increase over time (P=0.10). The ratio of BCL-2/BAX gene expression with approaching to lactation peak, showed a decreasing trend for the PO group but, for the FO group, an increase was observed indicating that apoptosis in mammary gland tissue was decreased. In conclusion, it is probable that the consumption of fish oil from mid dry period to lactation peak postpone the apoptosis process in Holstein cow’s mammary gland.
Keywords
gene expression
Holstein cow
mammary gland
n-3 fatty acids
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  • Receive Date 02 October 2017
  • Revise Date 27 April 2018
  • Accept Date 28 April 2018