Iranian Journal of animal Science

Iranian Journal of animal Science

Study of Genetic variation in Iranian Kurdish horse using microsatellite marker

Document Type : Research Paper

Authors
Motahareh Ala Amjadi 1
Hassan Mehrabani yegahneh 2
Mostafa Sadeghi 2
1 M. Sc. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Associat Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijas.2017.237799.653544
Abstract
In this research genetic variation  using  6  microsatellite  loci  (HMS07, HMS03, HMS02, HMS06, ASB02,  ASB23)  was studied in Iranian Kurdish horse population .Whole blood samples were randomly collected  from  52 horses in  Kurdistan, Kermanshah, Elam, Western Azerbaijan, Esfahan, Kerman and Hamadan regions. DNA was extracted from blood samples. Polymerase chain reaction (PCR) was used for amplification of 6   microsatellite loci using pairs of standard specific primers. Then, the PCR products assessed with 8% silver-staining acrylamide gel and electrophoresed. All microsatellites loci were polymorphic. The number of alleles observed and expected hetrozygosities were calculated with Shannon Index. The largest and smallest number of alleles were observed in HMS07 locus (10 alleles) and HMS02 (4 alleles) respectively. Our results confirmed the high efficiency of microsatellite markers for assessing population structure in Iranian native horses.
Keywords
Kurdish horse
microsatellite markers
polymorphisms
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Iranian Journal of animal Science
Volume 48, Issue 3 - Serial Number 3
Autumn 2017
Pages 335-342

  • Receive Date 05 August 2017
  • Revise Date 11 September 2017
  • Accept Date 24 September 2017