Iranian Journal of animal Science

Iranian Journal of animal Science

Study the polymorphism of Exon 4 of GH gene and exon 10 of GHR gene and their association with carcass traits in Lori-Bakhtiari breed sheep using PCR-SSCP

Document Type : Research Paper

Authors
Hossein Moradi Shahrbabak 1
Rasoul Khodabakhshzadeh 2
Mostafa Sadeghi 3
Amir TaheriYeganeh 4
1 Assistant Professors, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Fromer M.Sc. Student, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran
3 Associate Professor, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Former M. Sc. Student and Head of Animal Breeding Center of Ministry of Agriculture of IRAN
10.22059/ijas.2017.61672
Abstract
The growth hormone (GH) gene is a candidate for growth trait in farm animals and plays an important role in growth metabolism. Growth hormone can contribute in growth metabolism when it's receptor (GHR) are on cells target. The single nucleotide polymorphism was occurred in GH and GHR genes and that associated with carcass traits in Lori-Bakhtiari sheep breed with using PCR-SSCP. In this research, blood samples were collected from the left jugular vein from 152 Lori-Bakhtiari sheep breed. Genomic DNA was extracted from blood samples by using the salting-out procedure and PCR were used to amplify the regions are located in exon 4 (214 bp segments) of the ovine GH gene and exon 10 (218 bp segments) of the ovine GHR Gene. The single stranded conformation polymorphism (SSCP) patterns of PCR products were studied using acrylamide gel electrophoresis and silver-nitrate staining method. The result showed polymorphism in GH gene but any difference between banding patterns for GHR gene was not observed. The sequencing results showed the presence of 5 Single nucleotide polymorphism for GH gene in the studied population. No significant associations of the available genotypes in the exon 4 of the ovine GH Gene with carcass traits.
Keywords
Growth hormone gene
growth hormone receptor gene
Single nucleotide polymorphism
sequencing
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  • Receive Date 11 October 2015
  • Revise Date 13 December 2015
  • Accept Date 27 December 2015