Iranian Journal of animal Science

Iranian Journal of animal Science

Effects of different centrifugation protocols on pre and post-cooling sperm quality in Caspian horses

Document Type : Research Paper

Authors
Hoosahng Noori 1
Armin Towhidi 2
Majid Bahreyni 3
Tooba Nadri 4
1 Former M.Sc. Student, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Veterinary Expert, Animal Breeding Center of Iran, Karaj, Iran
4 Ph.D. Candidate, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijas.2017.138918.653396
Abstract
This study aimed to determine the effects of different centrifuging protocols (different rotation and time) on Caspian horse sperm characteristics. In this study, semen samples from four healthy adult Caspian pony stalian were collected using an artificial vagina and then diluted in milk phosphocaseinat based extender. Then semen samples were divided into seven equal parts, which part assigned to treatments including centrifugation treatments (500×g, 1000×g and 2000×g each for 4 and 7 minutes) and without centrifuge (control). Cooling of treatments were done at 0, 24 and 48 hours after centrifugation. Sperm motion characteristics, viability and percentages of healthy sperm were assessed. The results showed that motility, viability and percentages of healthy sperm reduced in 2000×g treatments (p<0.05). Stored sperm in 500×g and 1000×g treatments had better quality compared to control group (p<0.05). In addition, compared to 2000×g treatment, in 500×g and 1000×g treatments a less recovery rate was observed (p<0.05). In conclusion, Caspian horse's semen in treatment with centrifuging for 7 minutes at 1000 g, showed better result.
Keywords
Healthy
matility
quality evaluation
Sperm
viability
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Iranian Journal of animal Science
Volume 47, Issue 4 - Serial Number 4
Winter 2017
Pages 481-487

  • Receive Date 20 December 2015
  • Revise Date 28 September 2016
  • Accept Date 28 February 2017