Iranian Journal of animal Science

Iranian Journal of animal Science

Association of the BoLA-DRB3 alleles with peripheral blood mononuclear cell proliferation in response to Staphylococcusaureus

Document Type : Research Paper

Authors
Hadi Atashi 1
Mohammad Moradi Shar e Babak 2
Hassan Mehrabani-Yegane 3
Seyed Reza Miraei-Ashtiani 2
Ghodratollah Rahimi-Mianji
1 Former Ph.D. Student, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran and Associate Professor, Department of Animal Science, University of Shiraz, Iran
2 Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijas.2016.59792
Abstract
The aim of this study was to investigate the association of bovine leukocyte antigen-DRB3 alleles with peripheral blood mononuclear cell (PBMC) proliferation in response to Staphylococcus aureus. The animals included in this study (n=347) were approximately of same age and comprised of F2 Holstein-Friesian ´ Charolais (n = 155), Holstein-Friesian backcross (F0 Holstein-Friesian dams crossed with unrelated F1 sires, n = 60), Charolais backcross (F1 dams crossed with F0 Charolais sire, n=46) and pure Holstein-Friesian (n = 86).  A sequence-based typing method was used in order to determine the genotype of the animals at BoLA-DRB3 locus and a linear mixed model was used to evaluate the association of bovine leukocyte antigen-DRB3 alleles with peripheral blood mononuclear cell (PBMC) proliferation. Beside the BoLA-DRB3 alleles, fixed effects of genetic group and gender and random effect of sires were included into the statistical model. In this research, 27 alleles were found for BoLA-DRB3 gene. The results showed that alleles BoLA-DRB3*0101 and BoLA-DRB3*0902 significantly affected on the stimulation index of S. aureus–induced peripheral blood mononuclear cell proliferation (P<0.05). The results may be useful for investigating the biological mechanism of immune response against S. aureus.
Keywords
BoLA-DRB3
Mastitis
peripheral blood mononuclear cell proliferation
Staphylococcus aureus
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Iranian Journal of animal Science
Volume 47, Issue 3 - Serial Number 3
Autumn 2016
Pages 369-378

  • Receive Date 18 April 2016
  • Revise Date 21 September 2016
  • Accept Date 25 September 2016