M. BAGHERI; R MIRAIE- ASHTIANI; M MORADI-SHAHRBABAK; A PAKDEL; A NEJATI-JAVAREMI
Abstract
The Toll-like Receptor 4 (TLR4) is an innate immune protein on cell surfaces that identifies Lipopolysaccharide (LPS) of gram-negative bacteria. Genetic markers associated with innate responses during mastitis could help in selection of cattle to improve disease resistance. The objective of this study ...
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The Toll-like Receptor 4 (TLR4) is an innate immune protein on cell surfaces that identifies Lipopolysaccharide (LPS) of gram-negative bacteria. Genetic markers associated with innate responses during mastitis could help in selection of cattle to improve disease resistance. The objective of this study was to determine the association between TLR4 gene and clinical mastitis, using selective genotyping method. A total of 270 cows of two extreme groups namely: top clinical mastitis resistant vs. top susceptible ones were selected. For genotyping, the 2 methods of: PCR-RFLP and PCR-SSCP were made use of. Identified polymorphisms induced A-G in exon 2, and C-T in exon 3. Following sequencing, polymorphism effects on clinical mastitis were analyzed using logistic regression. The results indicated that TLR4 gene was significantly associated with clinical mastitis resistance.
Majid Pasandideh; Mohammadreza Mohammad-abadi; Alireza Torang; Ali Esmaeilizadeh kashkoueiyejh
Volume 42, Issue 3 , January 2011, , Pages 199-205
Abstract
Quantitative trait loci (QTL) affecting milk production traits on bovine chromosome 6 close to the OPN gene location have been identified through several whole genome scans. OPN is a phosphoprotein, synthesized in a variety of tissues and cells and secreted into body fluids. In this study, DNA was isolated ...
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Quantitative trait loci (QTL) affecting milk production traits on bovine chromosome 6 close to the OPN gene location have been identified through several whole genome scans. OPN is a phosphoprotein, synthesized in a variety of tissues and cells and secreted into body fluids. In this study, DNA was isolated from blood samples collected from 398 Holstein cows in Tehran and Esfahan provinces. DNA isolation was performed using salting out method. RFLP-PCR method was used for genotyping of SNP C>T at position 8514 in OPN gene. Frequencies of CC, CT and TT genotypes were estimated as 19, 57 and 24%, respectively. Hardy-Weinberg Equilibrium (HWE) was evaluated using ? square test. The genotypes in this locus deviated from HWE. In this study, significant associations were observed between c.8514C>T genotypes in OPN gene and FATP2X, PROPER305 traits. The SNP, showing signi?cant association with milk traits would provide an excellent opportunity for marker assisted selection programs in dairy cattle.
Saherreh Jowzi Shekalgoorani; AbdolAhad Shadparvar; Rasoul VaezTorshizi; Mohammad Moradi Shahrbabak
Volume 41, Issue 3 , December 2010, , Pages 223-229
Abstract
Average age distribution and generation interval were investigated in four different selection pathways. i.e., Sire of future Sires (SS), Sire of future Dams (SD), Dam of future Sires (DS) and Dam of future Dams (DD). Young Bulls pathway (YB) is also considered as a path that affects the generation interval ...
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Average age distribution and generation interval were investigated in four different selection pathways. i.e., Sire of future Sires (SS), Sire of future Dams (SD), Dam of future Sires (DS) and Dam of future Dams (DD). Young Bulls pathway (YB) is also considered as a path that affects the generation interval in SD pathway when using progeny testing program. Data were collected from Animal Breeding Center of Iran. Data were consisted of 584053 DD, 236628 DS, 345213 SD and 60 SS born from 1994 to 2008. Cow survival rates showed a linear diminishing trend (b=-0.0461) with a high R-square (98%) from one lactation to the next. Young Bulls' sperm usage was much higher than the usage of proved bulls' sperm due to the lower sperm price. This has led to a lower generation interval in DS pathway, when compared with that reported in other studies. On the other hand, percent of usage of local proved sire was very low compared with imported proved sperm. By using age distribution, obtained through different pathways, the generation intervals were recorded as 6.5, 7.5, 4.22, 4.10 and 3.37 years for SS, SD, DS, DD and YB pathways, respectively. Considering 20% test capacity, mean generation interval in Holstein dairy cattle in Iran was around 5.37. To overcome the problem of high generation interval, it is necessary to consider a suitable policy in selection/ culling and in a controlled use of young bulls’ semen.
Mohamad Saheb Honar; Mohamad Moradi Shahr Babak; Seyed Reza Miraei Ashtiani; Mohamad Bagher Sayad Nezhad
Volume 41, Issue 2 , September 2010
Abstract
Genetic trend for production traits were estimated in Iranian Holstein cattle using the first lactation data recorded by Cattle Breeding and Dairy Product Improvement Center of the State from 1991 to 2006. Effect of some factors on genetic trend was investigated using data from 18 large herds. (Co) variance ...
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Genetic trend for production traits were estimated in Iranian Holstein cattle using the first lactation data recorded by Cattle Breeding and Dairy Product Improvement Center of the State from 1991 to 2006. Effect of some factors on genetic trend was investigated using data from 18 large herds. (Co) variance components and genetic parameters were estimated, using five trait animal model and EM-AI algorithm in WOMBAT software. Estimated heritability for milk, fat, protein yields, fat percent and protein percent were 0.255, 0.197, 0.210, 0.317 and 0.308, respectively. Linear regression of Estimated Breeding Values (EBV) on birth year was used to estimate genetic trend. Estimated genetic trend for the animals for milk, fat and protein yield were 35.437±2.655, 0.714±0.059, 0.945±0.069 kg and for fat and protein percent were -0.006±0.0005 and -0.002±0.0002, (P
Ayatollah Chookani; Mohammad Dadpasand; HamidReza Mirzaei; Mohammad Rokouii; MohammadBagher SayadNezhad
Volume 40, Issue 4 , March 2010
bakhtiyar amini; mohammad moradi shahr babak; ardeshir nejati javaremi; mohammad bagher sayad nejad
Volume 40, Issue 2 , September 2009
Abstract
Adjusted milk yield for 305 days and two milking times along with actual first lactation records of 187138 Holstein cows were used to find out the effect of record adjustment on variance components estimation and on genetic evaluation. Records had been gathered by the Animal Breeding Center of Iran during ...
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Adjusted milk yield for 305 days and two milking times along with actual first lactation records of 187138 Holstein cows were used to find out the effect of record adjustment on variance components estimation and on genetic evaluation. Records had been gathered by the Animal Breeding Center of Iran during years 1991 to 2006. The pedigree file included 288032 animals. A single animal model with Average Information Restricted Maximum Likelihood (AIREML) was employed for data analysis. Milk yield heritability was estimated as 0.24 and 0.23 for adjusted and actual records, respectively. The rank correlations of 0.977, 0.975, and 0.987 were estimated for all, female, and male animals respectively based on the estimated breeding values of adjusted and non-adjusted records. The rank correlations among the 100, 200, 1000, and 2000 top animals were 0.500, 0.377, 0.491, and 0.529 respectively. In general, it could be concluded that adjustment of records tends to incline the breeding value of animals toward the population mean, leading to selection of animals of average breeding value.
Reza Tohidii; Rasoul Vaez torshizi; Mohammad Moradi shahr babak; mohammad bagher sayad nejad
Volume 39, Issue 1 , February 2009
Abstract
A pedigree file of 382831 Holstein cows and bulls, collected from herds registered by Animal Breeding Center of Iran during 1967 to 1999, was used to calculate the inbreeding coefficient. The mean inbreeding coefficient for the whole population (male and female), on a base year of 1946, was 0.184%. The ...
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A pedigree file of 382831 Holstein cows and bulls, collected from herds registered by Animal Breeding Center of Iran during 1967 to 1999, was used to calculate the inbreeding coefficient. The mean inbreeding coefficient for the whole population (male and female), on a base year of 1946, was 0.184%. The means for cows (94 percent of the whole population) and bulls (6 percent of the whole population) were 0.180% and 0.240%, respectively. Minimum inbreeding coefficient was zero, while the maximum values were 39.844, 39.844, and 25.780 for the whole population, cows and bull populations, respectively. In this population 9.5, percent of animals were inbred with a mean inbreeding coefficient of 2.175%. The regression coefficients of inbreeding on year for the whole population and for the population of cows were similar and positive (0.021%). This regression was positive for bulls but higher than that for the population of cows (0.026%).
