Reza Khalkhali-Evrigh; Nemat Hedayat-Evrigh; Hassan Hafezian; Ayoub Farhadi; Mohammad reza Bakhtiarizadeh
Abstract
The present study was performed to identify the copy number variations and their impacts on the genes of dromedary camels using whole genome sequencing data of two Iranian dromedary camels (Yazdi camel and Trodi camel). Whole genome sequencing of the Yazdi and Trodi samples produced about 456 and 418.8 ...
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The present study was performed to identify the copy number variations and their impacts on the genes of dromedary camels using whole genome sequencing data of two Iranian dromedary camels (Yazdi camel and Trodi camel). Whole genome sequencing of the Yazdi and Trodi samples produced about 456 and 418.8 paired-end reads with a read length of 100 bp, respectively. After mapping of trimmed reads to reference genome (NCBI accession number: GCA_000767585.1), a read-depth based algorithm was used to identify copy number variations. Identified copy number variations of studied samples, were 831 for Yazdi and 312 for Trodi camels. Nearly 60% (606 genes for Yazdi and 288 for Trodi camel) of the identified variants overlapped with the genes, and the rest were in the none genic regions. The obtained results showed that important genes including genes involved in immune system function and programmed cell death have copy number variation. As well as, two important genes of studied samples, OOEP and WWC3, which are involved in mammalian reproductive function, had copy number variation.
Amin Kazemi; Ahmad Zareh Shahneh; Saeed Zeinoaldini; Ali Reza Yousefi; Mehdi Heidari; Meysam Tavakoli-Alamooti; Zarbakhat Ansari
Abstract
This study was aimed to determine the effect of dietary Curcumin supplementation on the plasma lipid profile and some sperm quality parameters in broiler breeder roosters. In a completely randomized design, a total of twenty-eight 51-weeks-old Ross-308 roosters were randomly assigned to 4 treatment groups ...
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This study was aimed to determine the effect of dietary Curcumin supplementation on the plasma lipid profile and some sperm quality parameters in broiler breeder roosters. In a completely randomized design, a total of twenty-eight 51-weeks-old Ross-308 roosters were randomly assigned to 4 treatment groups (n=7) and individually caged for 9 successive weeks. Treatments were different levels of Curcumin that were added to a basal diet including: T1, control (no Curcumin supplement), T2, 0.006%; T3, 0.012%, and T4, 0.018% of the diet. To determine plasma lipid profile, blood samples were collected from five birds/treatment at the end of the trial. Also, semen samples were weekly collected from each bird during the experiment, and sperm motility and plasma membrane integrity were evaluated. The results showed that concentrations of the plasma glucose, triglyceride, total cholesterol, and LDL were decreased, and concentrations of HDL were increased in T3 and T4 groups compared to the control group (P < 0.05). There were no significant differences in plasma lipid profile and plasma concentration of glucose between T1 and the control group (P<0.05). Sperm plasma membrane integrity and motility were linearly improved in treated groups compared to the control (P<0.05). The highest decrease in plasma lipid profile and most improvements in sperm motility and plasma membrane integrity was observed in T4 groups compared with other groups. In conclusion, considering all the measured parameters, dietary supplementation of 0.018% Curcumin had the best response on modifying plasma lipid profiles and improving sperm quality characteristics compared with other treatments.
Fatemeh Saemi; Ahmad Zareh Shahneh; Mahdi Zhandi; Amir Akhlaghi; Sharif Rostami
Abstract
The aim of this experiment was to study the effects of long-term induced hyperthyroidism on the body weight and egg production. In addition the relationships between hyperthyroidism and some reproductive parameters were studied. A total of seventy 47-week-old Cobb 500 breeder hens were randomly allotted ...
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The aim of this experiment was to study the effects of long-term induced hyperthyroidism on the body weight and egg production. In addition the relationships between hyperthyroidism and some reproductive parameters were studied. A total of seventy 47-week-old Cobb 500 breeder hens were randomly allotted to two treatment groups (5 replicates of 7 hens each). Thyroxine was orally administered to the hyperthyroid group (0.3 mg/bird/day) for 100 consecutive days during which the body weight and egg production were recorded on a weekly basis. Effect of hyperthyroidism on plasma T4 levels was significant; but, the T3 level was not affected. Results indicated a significant decreasing effect of thyroid hormones on body weight. Thyroid hormones had no effect on egg production; however, the treatment × time (wk) interaction effect was significant on egg production. Ovarian and oviductal weight were not different between the control and hyperthyroid hens. Administration of thyroxine to breeder hens decreased the diameter of F1 follicles.
S. H. HOSSEINI; Y. ROOZBEHAN; A. R. AGHASHAHI; J. REZAI
Abstract
This research was conducted to assess the effect of substituting corn for barley grain in diet on performance and reproductive characteristics of early lactating, Holstein dairy cattle. A number of fifteen cows in their second lactation (26±11 DIM) with 36.7±5.6 kg milk/d were investigated ...
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This research was conducted to assess the effect of substituting corn for barley grain in diet on performance and reproductive characteristics of early lactating, Holstein dairy cattle. A number of fifteen cows in their second lactation (26±11 DIM) with 36.7±5.6 kg milk/d were investigated for 75 day (15 day for adaptation and 60 day for sampling) in a completely randomized design. The iso-energetic and iso-nitrogenous diets contained different levels (80, 160 or 240 g/kg DM) of corn substituted for barley grain. Feed was offered ad libitum as total mixed ration. In the experimental period, daily Dry Matter Intake (DMI), milk yield, body weight blood chemistry parameters, as well as reproductive characteristics were recorded. The DMI, milk yield, milk fat and protein percentage, plasma glucose and insulin concentrations, Services Per Conception(SPC) and Open Days (OD) for the cows fed the diet containing 80 g of corn/kg DM, respectively, were 24.76, 42.07 kg/d, 3.26 and 2.98%, 60.1 mg/dL, 0.409 µg/L, 1.6 and 95.8 d. Daily DMI, milk yield and feed efficiency were recorded as similar among treatments. Increasing the level of corn in the diet did not affect milk fat, protein and lactose, although the percentages of protein and fat were, numerically slightly increased. With increase in the level of corn in the diet, plasma concentration of insulin, numerically, increased, but beta hydroxyl butyrate and cholesterol declined, numerically. Replacing 240 g/kg of corn for barley in the diet caused a numerical decrease of SPC and OD as compared with other treatments. Overall, replacing corn grain for barley in the diet did not significanty affect milk production and composition. On the other hand, starch source did but only slightly affect the blood metabolites and reproductive parameters.
Mohamad Saheb Honar; Mohamad Moradi Shahr Babak; Seyed Reza Miraei Ashtiani; Mohamad Bagher Sayad Nezhad
Volume 41, Issue 2 , September 2010
Abstract
Genetic trend for production traits were estimated in Iranian Holstein cattle using the first lactation data recorded by Cattle Breeding and Dairy Product Improvement Center of the State from 1991 to 2006. Effect of some factors on genetic trend was investigated using data from 18 large herds. (Co) variance ...
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Genetic trend for production traits were estimated in Iranian Holstein cattle using the first lactation data recorded by Cattle Breeding and Dairy Product Improvement Center of the State from 1991 to 2006. Effect of some factors on genetic trend was investigated using data from 18 large herds. (Co) variance components and genetic parameters were estimated, using five trait animal model and EM-AI algorithm in WOMBAT software. Estimated heritability for milk, fat, protein yields, fat percent and protein percent were 0.255, 0.197, 0.210, 0.317 and 0.308, respectively. Linear regression of Estimated Breeding Values (EBV) on birth year was used to estimate genetic trend. Estimated genetic trend for the animals for milk, fat and protein yield were 35.437±2.655, 0.714±0.059, 0.945±0.069 kg and for fat and protein percent were -0.006±0.0005 and -0.002±0.0002, (P
