پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 56 2 2025 06 22 Effect of nano folic acid and folic acid on mortality rate and homocysteine concentration in broiler chickens Effect of nano folic acid and folic acid on mortality rate and homocysteine concentration in broiler chickens 317 332 99607 10.22059/ijas.2024.367860.653973 FA Ebrahim Shahraki Department of Animal Science, Faculty of Animal Science, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran 0009-0000-3980-2244 Mohammad Kazemifard Department of Animal Science, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. Mansour Rezaei Department of Animal Science, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. Zarbakht Ansari Pirsarai Department of Animal Science, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. Mahmood Barani Mycology and Medical Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran. Journal Article 2023 11 12 The aim of this study was to investigate and compare the effect of nano folic acid supplementation and folic acid in reducing serum homocysteine level, mortality rate and some blood biochemical parameters in broiler chickens. A total of 250 male broiler chickens of Ross 308 hybrid were implemented for 42 days as a completely randomized design in a 2x2 factorial format with five treatments and five replications and ten chickens in each replication. Treatments include: 1) control ration, 2) Folic acid-free diet with folic acid supplement (4 mg per liter of water) in non-nano form, 3) Folic acid-free diet with folic acid supplement (4 mg per liter of water) in nano form, 4) control diet with folic acid supplement (4 mg per liter of water) in non-nano form, 5) Control diet with folic acid supplement (4 mg per liter of water) were in nano form. After six weeks, serum samples were collected and analyzed for homocysteine concentration. The results showed that regular folic acid significantly decreased homocysteine concentration in serum and decreased mortality (P<0.05), but in nano folic acid treatment, this reduction was more than other treatments. The interaction effect of nano folic acid and regular folic acid supplements significantly reduced serum homocysteine levels compared to the control (P<0.05). But in the control treatment, it had the highest level of homocysteine concentration and the highest mortality. Also, nano folic acid and folic acid significantly decreased the activity of alanine aminotransferase and aspartate aminotransferase compared to the control treatment (P<0.05). But it had no significant effect on the activity of alkaline phosphatase. In addition, folic acid and nano folic acid significantly increased the concentration of thyroid hormones (T3 and T4) compared to the control treatment (P<0.05). Therefore, we conclude that nano folic acid is more effective than normal folic acid in reducing the concentration of homocysteine in blood circulation and may be a promising alternative to optimize homocysteine metabolism and reduce cardiovascular risks associated with mortality in broilers. The aim of this study was to investigate and compare the effect of nano folic acid supplementation and folic acid in reducing serum homocysteine level, mortality rate and some blood biochemical parameters in broiler chickens. A total of 250 male broiler chickens of Ross 308 hybrid were implemented for 42 days as a completely randomized design in a 2x2 factorial format with five treatments and five replications and ten chickens in each replication. Treatments include: 1) control ration, 2) Folic acid-free diet with folic acid supplement (4 mg per liter of water) in non-nano form, 3) Folic acid-free diet with folic acid supplement (4 mg per liter of water) in nano form, 4) control diet with folic acid supplement (4 mg per liter of water) in non-nano form, 5) Control diet with folic acid supplement (4 mg per liter of water) were in nano form. After six weeks, serum samples were collected and analyzed for homocysteine concentration. The results showed that regular folic acid significantly decreased homocysteine concentration in serum and decreased mortality (P<0.05), but in nano folic acid treatment, this reduction was more than other treatments. The interaction effect of nano folic acid and regular folic acid supplements significantly reduced serum homocysteine levels compared to the control (P<0.05). But in the control treatment, it had the highest level of homocysteine concentration and the highest mortality. Also, nano folic acid and folic acid significantly decreased the activity of alanine aminotransferase and aspartate aminotransferase compared to the control treatment (P<0.05). But it had no significant effect on the activity of alkaline phosphatase. In addition, folic acid and nano folic acid significantly increased the concentration of thyroid hormones (T3 and T4) compared to the control treatment (P<0.05). Therefore, we conclude that nano folic acid is more effective than normal folic acid in reducing the concentration of homocysteine in blood circulation and may be a promising alternative to optimize homocysteine metabolism and reduce cardiovascular risks associated with mortality in broilers. https://ijas.ut.ac.ir/article_99607_6a12a0b561b4303f128248cb46457d97.pdf