پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 55 4 2024 12 21 The Effect of Feeding Top-Dress Cottonseed Bioactive Peptide and Organic Selenium in the Prepartum on the Health of the Reproductive Tract and Subsequent Pregnancy in Holstein Dairy Cattle The Effect of Feeding Top-Dress Cottonseed Bioactive Peptide and Organic Selenium in the Prepartum on the Health of the Reproductive Tract and Subsequent Pregnancy in Holstein Dairy Cattle 727 751 97799 10.22059/ijas.2024.373793.654006 FA Zeinab Mirbagheri Marvili Department of Animal Science, Agriculture Faculty, University of Zanjan., Zanjan. Iran. 0009-0001-9098-4132 Hamid Amanlou Department of animal science, Faculty of agriculture, University of Zanjan, Zanjan, Iran 0000-0002-5631-125X Najme Eslamian Farsuni Agriculture and natural resources research and education center of Chahar Mahal and Bakhtiari province, Animal science research, Iran 0000-0002-7921-3302 Journal Article 2024 03 14 The aim of this study was to investigate the effect of feeding top dress Hydroxy Seleno Methionine (HSM) as organic Selenium and cotton seed bioactive peptide (CSBP) on body condition, health of the reproductive system and subsequent pregnancy of dairy cows. For this purpose, one hundred and eighty multiparous Holstein cows at the day 21 prior to the expected calving date were assigned to one of four experimental treatments in a randomized complete block design in a 2x2 factorial arrangement: 1) Basal diet (containing mineral selenium as per NRC (2001) recommendations without peptide; 2) Basal diet plus 300 g bioactive peptide; 3) Basal diet plus 1.2 mg organic selenium per kg of dry matter without bioactive peptides; 4) Basal diet plus 1.2 mg organic selenium per kg of dry matter plus 300 grams of bioactive peptide. The inclusion of HSM during the prepartum period resulted in a higher BCS in postpartum and smaller increase in BCS during prepartumd (P < 0.01). Additionally, there was a significant interaction effect between HSM and CSBP on BCS (P < 0.02) and its changes (P < 0.01) during the post-partum periods. The presence of ovarian cysts, number of inseminations per pregnancy, the endometrium and uterine regression scores and pregnancy during the first insemination was not influenced by any of the experimental treatments (P > 0.1). However, HSM had a positive effect on pregnancy during the second insemination (P < 0.05). It can be concluded that supplementing HSM could reduce the BCS changes before and after parturition and  subsequent to that, it leads to an enhancement in the pregnancy during the second insemination. The aim of this study was to investigate the effect of feeding top dress Hydroxy Seleno Methionine (HSM) as organic Selenium and cotton seed bioactive peptide (CSBP) on body condition, health of the reproductive system and subsequent pregnancy of dairy cows. For this purpose, one hundred and eighty multiparous Holstein cows at the day 21 prior to the expected calving date were assigned to one of four experimental treatments in a randomized complete block design in a 2x2 factorial arrangement: 1) Basal diet (containing mineral selenium as per NRC (2001) recommendations without peptide; 2) Basal diet plus 300 g bioactive peptide; 3) Basal diet plus 1.2 mg organic selenium per kg of dry matter without bioactive peptides; 4) Basal diet plus 1.2 mg organic selenium per kg of dry matter plus 300 grams of bioactive peptide. The inclusion of HSM during the prepartum period resulted in a higher BCS in postpartum and smaller increase in BCS during prepartumd (P < 0.01). Additionally, there was a significant interaction effect between HSM and CSBP on BCS (P < 0.02) and its changes (P < 0.01) during the post-partum periods. The presence of ovarian cysts, number of inseminations per pregnancy, the endometrium and uterine regression scores and pregnancy during the first insemination was not influenced by any of the experimental treatments (P > 0.1). However, HSM had a positive effect on pregnancy during the second insemination (P < 0.05). It can be concluded that supplementing HSM could reduce the BCS changes before and after parturition and  subsequent to that, it leads to an enhancement in the pregnancy during the second insemination. https://ijas.ut.ac.ir/article_97799_6be4eb713cbecc8db63e9a2e92dd7dc1.pdf