پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 55 4 2024 12 21 Efficiency of genotyping by sequencing technology and its comparison with single nucleotide polymorphism array in an F2 chickens population Efficiency of genotyping by sequencing technology and its comparison with single nucleotide polymorphism array in an F2 chickens population 693 711 97795 10.22059/ijas.2024.372827.653999 FA Narjes Gorgani Firouzjah PhD student,, Department of Animal Science, Agricultural Faculty, Tarbiat Modares University, Tehran,, Iran. Rasoul Vaez Torshizi Department of Animal Science, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran. Alireza Ehsani Department of Animal Science, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran. Ali Akbar Masoudi Department of Animal Science, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran. Peymaneh Davoodi Department of Animal Science, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran. Hossein Bani Saadat Department of Animal Science, Agricultural Faculty, Tarbiat Modares University, Tehran, Iran. 0000-0001-9034-0372 Journal Article 2024 02 19 For researches, there is a wide variety of available technologies to collect molecular information in the field of chicken genomics. These technologies, which consist of single-nucleotide polymorphism (SNP) arrays and genotyping by sequencing (GBS), depending on the goals of the study, can have different applications. The aim of this study was to compare the results of markers genotyped by two technologies, namely, 60 K SNP BeadChip and genotyping by sequencing, using data collected on F₂ chicken population resulting from a reciprocal crosses between a native bird of Urmia and a fast-growing commercial Arian line. In genotyping by GBS, 882,918 SNPs were identified, of which 815,613 SNPs (92.40%) were located on chromosomes 1 to 28. In 60 K SNP array, the number of SNPs for each sample were 51347, which were distributed on chromosomes 1 to 28. The GBS data identified more markers and haplotype blocks than the 60 K SNP array. The rate of linkage disequilibrium (LD) in the physical distances of 10, 100 and 1000 kbp in GBS was less than that of SNP array. The large variety of SNPs in the GBS resulted in a uniform population structures and kinship. Also, in addition to the high performance for identifying single nucleotide polymorphisms, the technology of GBS also reduced the costs of the genotyping for each sample, therefore, it seems that the use of genotyping by sequencing technology could be a suitable alternative method to the 60 K SNP BeadChip array technology for genome-wide association studies in chicken population.     For researches, there is a wide variety of available technologies to collect molecular information in the field of chicken genomics. These technologies, which consist of single-nucleotide polymorphism (SNP) arrays and genotyping by sequencing (GBS), depending on the goals of the study, can have different applications. The aim of this study was to compare the results of markers genotyped by two technologies, namely, 60 K SNP BeadChip and genotyping by sequencing, using data collected on F₂ chicken population resulting from a reciprocal crosses between a native bird of Urmia and a fast-growing commercial Arian line. In genotyping by GBS, 882,918 SNPs were identified, of which 815,613 SNPs (92.40%) were located on chromosomes 1 to 28. In 60 K SNP array, the number of SNPs for each sample were 51347, which were distributed on chromosomes 1 to 28. The GBS data identified more markers and haplotype blocks than the 60 K SNP array. The rate of linkage disequilibrium (LD) in the physical distances of 10, 100 and 1000 kbp in GBS was less than that of SNP array. The large variety of SNPs in the GBS resulted in a uniform population structures and kinship. Also, in addition to the high performance for identifying single nucleotide polymorphisms, the technology of GBS also reduced the costs of the genotyping for each sample, therefore, it seems that the use of genotyping by sequencing technology could be a suitable alternative method to the 60 K SNP BeadChip array technology for genome-wide association studies in chicken population.     https://ijas.ut.ac.ir/article_97795_04099638c4c23447c521c1b4a34f9c60.pdf