پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 54 4 2023 11 22 Effect of selenium nanoparticles on quantitative and qualitative parameters of dog sperm after storage in the refrigerator Effect of selenium nanoparticles on quantitative and qualitative parameters of dog sperm after storage in the refrigerator 379 390 95184 10.22059/ijas.2023.350293.653914 FA Mostafa Panahi Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. 0000-0003-2335-4963 Ali Soleimanzadeh Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. 0000-0002-1591-2198 Ali Shalizar-Jalali Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. 0000-0002-9811-7754 Esmail Ayen Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. 0000-0002-4204-8163 Rahim Molaie Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. 0000-0002-0107-1164 Journal Article 2022 10 24 Sperm cryopreservation has become an indispensable tool in reproductive biology. However, maintaining fertility in frozen sperm is very important. The aim of this study is to investigate the effects of selenium nano-particles on the storage of dog semen at 4 ˚C during a period of 48 hours. In this study, 20 ejaculates were collected from 4 mix dogs and diluted in a Tris-based diluent. Then, they were divided into 4 parts in control and treated with 0.5, 1 and 1.5 µg selenium nano-particles groups. Sperm parameters including evaluation of total and progressive motility sperm, motility characteristics and viability of sperm were evaluated for 48 hours. The obtained results showed that in concentrations of 1 µg [progressive sperm (27.03±1.48), total motility (49.48±1.27) and viability (41.40±1.03)] and 0.5 µg [progressive sperm (30.76±1.67), total motility (53.18±1.54) and viability (43.15±1.58)] of selenium nanoparticles compared to the control group, but in the concentration of 1.5 µg was lower than the control group (p< 0.05). As a result, the present study showed that adding selenium nanoparticles in concentrations of 0.1 and 0.5 µg to dog semen can improve the parameters of dog semen after liquid storage, but in concentrations of 1.5 µg it causes toxic effects and overall agitation. And it reduces sperm viability. Sperm cryopreservation has become an indispensable tool in reproductive biology. However, maintaining fertility in frozen sperm is very important. The aim of this study is to investigate the effects of selenium nano-particles on the storage of dog semen at 4 ˚C during a period of 48 hours. In this study, 20 ejaculates were collected from 4 mix dogs and diluted in a Tris-based diluent. Then, they were divided into 4 parts in control and treated with 0.5, 1 and 1.5 µg selenium nano-particles groups. Sperm parameters including evaluation of total and progressive motility sperm, motility characteristics and viability of sperm were evaluated for 48 hours. The obtained results showed that in concentrations of 1 µg [progressive sperm (27.03±1.48), total motility (49.48±1.27) and viability (41.40±1.03)] and 0.5 µg [progressive sperm (30.76±1.67), total motility (53.18±1.54) and viability (43.15±1.58)] of selenium nanoparticles compared to the control group, but in the concentration of 1.5 µg was lower than the control group (p< 0.05). As a result, the present study showed that adding selenium nanoparticles in concentrations of 0.1 and 0.5 µg to dog semen can improve the parameters of dog semen after liquid storage, but in concentrations of 1.5 µg it causes toxic effects and overall agitation. And it reduces sperm viability. https://ijas.ut.ac.ir/article_95184_35dce29cf91a19ead1de07e2c3616bd1.pdf