پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 54 4 2023 11 22 Effect of injection of zinc oxide nano particles on the immune response, oxidative enzyme activity of plasma and gene expression in broilers spleen tissue Effect of injection of zinc oxide nano particles on the immune response, oxidative enzyme activity of plasma and gene expression in broilers spleen tissue 391 402 94453 10.22059/ijas.2022.348850.653908 FA Mohammad Kazemifard Department of Animal science, Faculty of animal and fisheries sciences, Sari agricultural and natural resources university, Sari, Iran 0000-0002-1880-343X Mansour Rezaei Department of Animal science, Faculty of animal and fisheries sciences, Sari agricultural and natural resources university, Sari, Iran 0000-0003-0515-6365 جوانشیر پالوج Department of Animal science, Faculty of animal and fisheries sciences, Sari agricultural and natural resources university, Sari, Iran 0000-0001-5642-7175 Zarbakht Ansari Pirsaraei Department of Animal science, Faculty of animal and fisheries sciences, Sari agricultural and natural resources university, Sari, Iran 0000-0003-2589-1463 Essa Dirandeh Department of Animal science, Faculty of animal and fisheries sciences, Sari agricultural and natural resources university, Sari, Iran 0000-0002-0773-3966 Journal Article 2022 09 20 This experiment was conducted to investigate the effect of zinc oxide nanoparticls injection on immune response and performance in broilers. One hundred eight Ross308 strain chicks were injected in a completely randomized design with three treatments in four replicates and nine chicks in each replicate on the day of hatching. The treatments include: 1. Control, 2. Injection of 20 microliters of zinc oxide nanoparticls (0.5 mg of zinc oxide nanoparticls in 50 ml of saline), 3. Injection of 20 microliters of zinc oxide nanoparticles (0.6 mg of zinc oxide nanoparticles in 50 ml of saline). The zinc oxide nanoparticls solution was injected into the chickens from the neck area. The ration used for all treatments was the standard ration of Ross 308 strain. Influenza antibody titer 14 days and CBH test, 12 and 24 hours after vaccination and glutathione peroxidase activity level increased significantly in treatment 3 compared to other treatments (P<0.05). Interleukin 12 gene expression in treatment 2 and 3 was significantly higher than the control treatment (P<0.05). The relative weight of gravel in treatment 3 compared to the control treatment and the feed consumed in treatments 2 and 3 were significantly lower than the control treatment (P<0.05). The results showed that the injection of zinc oxide nanoparticls increased the general and cellular immune response, the activity of antioxidant enzymes and interleukin 12 gene expression. This experiment was conducted to investigate the effect of zinc oxide nanoparticls injection on immune response and performance in broilers. One hundred eight Ross308 strain chicks were injected in a completely randomized design with three treatments in four replicates and nine chicks in each replicate on the day of hatching. The treatments include: 1. Control, 2. Injection of 20 microliters of zinc oxide nanoparticls (0.5 mg of zinc oxide nanoparticls in 50 ml of saline), 3. Injection of 20 microliters of zinc oxide nanoparticles (0.6 mg of zinc oxide nanoparticles in 50 ml of saline). The zinc oxide nanoparticls solution was injected into the chickens from the neck area. The ration used for all treatments was the standard ration of Ross 308 strain. Influenza antibody titer 14 days and CBH test, 12 and 24 hours after vaccination and glutathione peroxidase activity level increased significantly in treatment 3 compared to other treatments (P<0.05). Interleukin 12 gene expression in treatment 2 and 3 was significantly higher than the control treatment (P<0.05). The relative weight of gravel in treatment 3 compared to the control treatment and the feed consumed in treatments 2 and 3 were significantly lower than the control treatment (P<0.05). The results showed that the injection of zinc oxide nanoparticls increased the general and cellular immune response, the activity of antioxidant enzymes and interleukin 12 gene expression. https://ijas.ut.ac.ir/article_94453_db7153cd33dfe7c9d20694adabfd339c.pdf