پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 52 2 2021 08 23 Effect of diluted semen temperature and sperm concentration on sperm penetration ‎rate, fertility and hatchability of aged broiler breeder hens Effect of diluted semen temperature and sperm concentration on sperm penetration ‎rate, fertility and hatchability of aged broiler breeder hens 133 140 84027 10.22059/ijas.2020.307142.653792 FA Hossein Sharideh Researcher of ETKA Organization ‎ Ph.D. Graduate in Animal Physiology, Department of Animal Science, College of Agriculture and Natural ‎Resources, University of Tehran, Karaj, Iran Mojtaba Zaghari Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran 0000-0003-4440-3584 Seyed Jafar Mirbehbahani Researcher of ETKA Organization ‎ Journal Article 2020 08 02 The current study aimed to optimize artificial insemination in aged broiler breeder hens in two experiments. In the first experiment, the effect of (two) different diluted semen temperatures (5 and 25 °C) of Hubbard rooster (40 roosters, 58 weeks of age) on fertility, hatchability and sperm penetration (SP) rate in the perivitelline layer of Hubbard hen (180 hens) were investigated. In the second experiment, three (different) sperm concentrations (100 (C100), 200 (C200), and 400 (C400) million sperm in 0.25 mL per hen) of Hubbard roosters (40 roosters, 62 weeks of age) on fertility, hatchability and SP rate of Hubbard broiler breeder hens (270 hens) were explored. In the first experiment, the results showed that the temperature of 5 °C of diluted semen compared to the 25 °C, increased percentage of hatchability of set eggs, hatchability of fertile eggs, and SP and decreased early embryonic mortality. The results of the second experiment showed the highest percentage of fertility and SP rate were observed at treatment C400. Also, in this experiment that highest percentage of hatchability of set eggs and hatchability of fertile eggs and lowest early embryonic mortality were observed at treatment C400. Return on investment (ROI) of the treatments C200 and C400 was approximately 2.9 and 1.4, respectively. In overall, the results of this study showed that (in attention to ROI and hatchability) to optimize artificial insemination of aged broiler breeder hens we can use a sperm concentration of 200 to 400 million in 0.25 mL per hens at 5 °C. The current study aimed to optimize artificial insemination in aged broiler breeder hens in two experiments. In the first experiment, the effect of (two) different diluted semen temperatures (5 and 25 °C) of Hubbard rooster (40 roosters, 58 weeks of age) on fertility, hatchability and sperm penetration (SP) rate in the perivitelline layer of Hubbard hen (180 hens) were investigated. In the second experiment, three (different) sperm concentrations (100 (C100), 200 (C200), and 400 (C400) million sperm in 0.25 mL per hen) of Hubbard roosters (40 roosters, 62 weeks of age) on fertility, hatchability and SP rate of Hubbard broiler breeder hens (270 hens) were explored. In the first experiment, the results showed that the temperature of 5 °C of diluted semen compared to the 25 °C, increased percentage of hatchability of set eggs, hatchability of fertile eggs, and SP and decreased early embryonic mortality. The results of the second experiment showed the highest percentage of fertility and SP rate were observed at treatment C400. Also, in this experiment that highest percentage of hatchability of set eggs and hatchability of fertile eggs and lowest early embryonic mortality were observed at treatment C400. Return on investment (ROI) of the treatments C200 and C400 was approximately 2.9 and 1.4, respectively. In overall, the results of this study showed that (in attention to ROI and hatchability) to optimize artificial insemination of aged broiler breeder hens we can use a sperm concentration of 200 to 400 million in 0.25 mL per hens at 5 °C. https://ijas.ut.ac.ir/article_84027_3ff2f5a57bfb30a17ce89c58c2736866.pdf