پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 51 2 2020 07 22 Identification the copy number variation and its impacts on the genes of Iranian dromedary ‎camels using whole genome sequencing data Identification the copy number variation and its impacts on the genes of Iranian dromedary ‎camels using whole genome sequencing data 113 119 77438 10.22059/ijas.2020.294708.653761 FA Reza Khalkhali-Evrigh Former Ph.D. Student, Department of Animal Science, Faculty of Animal Science and Fisheries, Sari ‎Agricultural Sciences and Natural Resources University, Sari, Iran Nemat Hedayat-Evrigh Associate Professor, Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh ‎Ardabili, Ardebil, Iran Hassan Hafezian Associate Professor, Department of Animal Science, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and ‎Natural Resources University, Sari, Iran Ayoub Farhadi Associate Professor, Department of Animal Science, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and ‎Natural Resources University, Sari, Iran Mohammad Reza Bakhtiarizadeh Associate Professor, Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Iran Journal Article 2019 12 29 The present study was performed to identify the copy number variations and their impacts on the genes of dromedary camels using whole genome sequencing data of two Iranian dromedary camels (Yazdi camel and Trodi camel). Whole genome sequencing of the Yazdi and Trodi samples produced about 456 and 418.8 paired-end reads with a read length of 100 bp, respectively. After mapping of trimmed reads to reference genome (NCBI accession number: GCA_000767585.1), a read-depth based algorithm was used to identify copy number variations. Identified copy number variations of studied samples, were 831 for Yazdi and 312 for Trodi camels. Nearly 60% (606 genes for Yazdi and 288 for Trodi camel) of the identified variants overlapped with the genes, and the rest were in the none genic regions. The obtained results showed that important genes including genes involved in immune system function and programmed cell death have copy number variation. As well as, two important genes of studied samples, OOEP and WWC3, which are involved in mammalian reproductive function, had copy number variation. The present study was performed to identify the copy number variations and their impacts on the genes of dromedary camels using whole genome sequencing data of two Iranian dromedary camels (Yazdi camel and Trodi camel). Whole genome sequencing of the Yazdi and Trodi samples produced about 456 and 418.8 paired-end reads with a read length of 100 bp, respectively. After mapping of trimmed reads to reference genome (NCBI accession number: GCA_000767585.1), a read-depth based algorithm was used to identify copy number variations. Identified copy number variations of studied samples, were 831 for Yazdi and 312 for Trodi camels. Nearly 60% (606 genes for Yazdi and 288 for Trodi camel) of the identified variants overlapped with the genes, and the rest were in the none genic regions. The obtained results showed that important genes including genes involved in immune system function and programmed cell death have copy number variation. As well as, two important genes of studied samples, OOEP and WWC3, which are involved in mammalian reproductive function, had copy number variation. https://ijas.ut.ac.ir/article_77438_478288afaf4e7d55df3df6646f185a38.pdf