پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 51 2 2020 07 22 Effect of different levels of encapsulated glutathione on cryopreservation of bull ‎sperm Effect of different levels of encapsulated glutathione on cryopreservation of bull ‎sperm 91 101 77435 10.22059/ijas.2020.285074.653720 FA Touba Nadri Ph.D. Candidate of Animal Physiology, Department of Animal Science, College of Agriculture ‎and Natural Resources, University of Tehran, Karaj, Iran Saeed Zeinoaldini Associate Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of ‎Tehran, Karaj, Iran Armin Towhidi Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran Gholamhossein Riazi Professor, IBB Center, University of Tehran, Tehran, Iran Mahdi Zhandi Associate Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of ‎Tehran, Karaj, Iran Mohsen Sharafi Assistant Professor of Animal Physiology, Department of Animal Science, Tarbiat Modares University, Tehran, Iran Journal Article 2019 07 21 This study was designed to evaluate the effects of encapsulated reduced glutathione added to extender on the quality of bull sperm after freezing and thawing process. Extender contains lecithin nanoliposomes supplemented with different concentrations of glutathione (0, 1, 2.5 and 5 Mm concentration). The supplemented nanoliposomes with glutathione were prepared by thin-film preparation method.  The particle size decreased to nanometer by sonication. 40 ejaculations were collected from 6 Holstein bulls and frozen for 6 weeks. Evaluated characteristics after freezing and thawing process were kinetic parameters (CASA), membrane activity (HOST), membrane integrity (eosin-nigrosin) and morphology of sperm ( Hancock's solution). Results showed that level 1 mM significantly increased the total and progressive motility (47.5±1.7, 32.7±2 %).  Although, there was no significant difference with level 2.5 mM glutathione (45.0±1.7, 26.2±2 %). Also, the concentration of 2.5 mM showed the highest value of cell membrane integrity and cell membrane functionality but did not differ with the concentration of 1mM. However, supplementation of nanoliposomes with 5 mM glutathione reduced the quality of post-thaw sperm. Thus, level 1 M encapsulated glutathione can provide better protection compare to the other level of that for bull sperm. This study was designed to evaluate the effects of encapsulated reduced glutathione added to extender on the quality of bull sperm after freezing and thawing process. Extender contains lecithin nanoliposomes supplemented with different concentrations of glutathione (0, 1, 2.5 and 5 Mm concentration). The supplemented nanoliposomes with glutathione were prepared by thin-film preparation method.  The particle size decreased to nanometer by sonication. 40 ejaculations were collected from 6 Holstein bulls and frozen for 6 weeks. Evaluated characteristics after freezing and thawing process were kinetic parameters (CASA), membrane activity (HOST), membrane integrity (eosin-nigrosin) and morphology of sperm ( Hancock's solution). Results showed that level 1 mM significantly increased the total and progressive motility (47.5±1.7, 32.7±2 %).  Although, there was no significant difference with level 2.5 mM glutathione (45.0±1.7, 26.2±2 %). Also, the concentration of 2.5 mM showed the highest value of cell membrane integrity and cell membrane functionality but did not differ with the concentration of 1mM. However, supplementation of nanoliposomes with 5 mM glutathione reduced the quality of post-thaw sperm. Thus, level 1 M encapsulated glutathione can provide better protection compare to the other level of that for bull sperm. https://ijas.ut.ac.ir/article_77435_670a0bf5da6cdce4e756948e60ab7ef0.pdf