پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 47 4 2017 02 19 The Effect of Intra Amniotic Injection of Zinc-Methionine and Nano Zinc-Methionine on Metallothionein Gene Expression in the Broiler chickens The Effect of Intra Amniotic Injection of Zinc-Methionine and Nano Zinc-Methionine on Metallothionein Gene Expression in the Broiler chickens 621 632 61167 10.22059/ijas.2017.213018.653459 FA Kolsoom Razani Ph.D. Student, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran Majid Mottaghitalab Associate Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran Seyed Hosein Heseini Moghaddam Assistant Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran Journal Article 2016 08 03 The effects of <em>intra </em>amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein mRNA expression of chicken’s liver and intestine were studied. Four hundred fertiled eggs (Ross 308 strain) were allocated to 4 treatments, 4 replicates/ treatment and 25 eggs per replicate using a CRD (Complete Random Design) design. Experimental treatments were: 1- no injection (negative control), 2- injection of 1 ml 0.9% saline solution (positive control), 3- injection of 1 ml 25% zinc- methionine solution and 4- injection of 1 ml 25% nano-zinc- methionine solution. On day 17 of incubation, eggs were injected using insulin syringes. Results showed that <em>in Ovo </em>injection lead to no significant effect on hatchability. The injection of zinc-methionine and nano zinc-methionine caused significant (P<0.01) increase in chicks weight at hatch, and a week after that. A similar result was obtained for liver and intestine samples. Groups treated with zinc-methionine and nano zinc-methionine showed significant (P<0.01) increase in metallothionein mRNA expression in liver and intestine and remained significant (P<0.01) at day 7 after hatch. Experimental treatments significantly (P<0.01) increased the liver and intestine metallothionein content at day 1 and 7 after hatch. Conclusion is that in ovo injection of zinc-methionine and nano zinc-methionine lead to increase body weight at first 3weeks of broiler rearing. The effects of <em>intra </em>amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein mRNA expression of chicken’s liver and intestine were studied. Four hundred fertiled eggs (Ross 308 strain) were allocated to 4 treatments, 4 replicates/ treatment and 25 eggs per replicate using a CRD (Complete Random Design) design. Experimental treatments were: 1- no injection (negative control), 2- injection of 1 ml 0.9% saline solution (positive control), 3- injection of 1 ml 25% zinc- methionine solution and 4- injection of 1 ml 25% nano-zinc- methionine solution. On day 17 of incubation, eggs were injected using insulin syringes. Results showed that <em>in Ovo </em>injection lead to no significant effect on hatchability. The injection of zinc-methionine and nano zinc-methionine caused significant (P<0.01) increase in chicks weight at hatch, and a week after that. A similar result was obtained for liver and intestine samples. Groups treated with zinc-methionine and nano zinc-methionine showed significant (P<0.01) increase in metallothionein mRNA expression in liver and intestine and remained significant (P<0.01) at day 7 after hatch. Experimental treatments significantly (P<0.01) increased the liver and intestine metallothionein content at day 1 and 7 after hatch. Conclusion is that in ovo injection of zinc-methionine and nano zinc-methionine lead to increase body weight at first 3weeks of broiler rearing. https://ijas.ut.ac.ir/article_61167_626bc1a4d43866a4b77a2f25c3e42211.pdf