پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 47 3 2016 11 21 Improvement of broiler chickens immune system by in ovo injection of 25(OH) D3 and K3 vitamins Improvement of broiler chickens immune system by in ovo injection of 25(OH) D3 and K3 vitamins 349 356 59790 10.22059/ijas.2016.59790 FA Tahereh Abbasi M.Sc. Student, Department of Animal Science, Faculty of Agricultural Science & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran Malak Shakeri Assistant Professors, Department of Animal Science, Faculty of Agricultural Science & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran Mojtaba Zaghari Professor, Department of Animal Science, Faculty of Agricultural Science & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran Hamid Kohram Assistant Professor, Department of Animal Science, Faculty of Agricultural Science & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran Journal Article 2016 01 31 This experiment was conducted to determin the effect of 25(H)D₃ and K₃ vitamins on immune system of Ross 308 broiler chicken in a completely randomized design with a 3×4 factorial arrangement with four replicates and 16 observation per replicate. Treatments included vitamine D₃ (0, 0.4, 0.6 and 0.8 μg) and vitamin K₃ (0, 2 and 6 μg). On day 180 g incubation 0.5 ml of treatment solution was injucted in fertile eggs. Male chicken were reared in experiment cages for a period of 42 days. To evaluate humoral immune system reaction 0.5 ml SRBC (10%) was injected on chickens’ breast muscle at the 28th and 35th day, thereafter chicken blood samples were taken on the 35th and 42nd day. In order to evaluate the cellular immune system reaction, Phyto-hemagglutinin (PHA) was injected in the curtain chickens toe on day 24 of rearing period and skin response were studied on 1 and 5 days after injection. The results showed that with increasing levels of vitamin K₃, antibody titers against Newcastle were increased (P<0.05). Treatment of increased 0.4 μg and 6 μg K₃ in antibody levels to SRBC and IgG significantly and showed the maximum stimulation to PHA injection and treatment 12 with 6 μg K₃ and 0.8 μg D₃ showed the lowest amount of stimulation (p<0.05). Finally, it can be concluded that 0.4 μg vit D₃ and 6 μg vit K₃ can improve cellular and humoral immune response in broiler chickens. This experiment was conducted to determin the effect of 25(H)D₃ and K₃ vitamins on immune system of Ross 308 broiler chicken in a completely randomized design with a 3×4 factorial arrangement with four replicates and 16 observation per replicate. Treatments included vitamine D₃ (0, 0.4, 0.6 and 0.8 μg) and vitamin K₃ (0, 2 and 6 μg). On day 180 g incubation 0.5 ml of treatment solution was injucted in fertile eggs. Male chicken were reared in experiment cages for a period of 42 days. To evaluate humoral immune system reaction 0.5 ml SRBC (10%) was injected on chickens’ breast muscle at the 28th and 35th day, thereafter chicken blood samples were taken on the 35th and 42nd day. In order to evaluate the cellular immune system reaction, Phyto-hemagglutinin (PHA) was injected in the curtain chickens toe on day 24 of rearing period and skin response were studied on 1 and 5 days after injection. The results showed that with increasing levels of vitamin K₃, antibody titers against Newcastle were increased (P<0.05). Treatment of increased 0.4 μg and 6 μg K₃ in antibody levels to SRBC and IgG significantly and showed the maximum stimulation to PHA injection and treatment 12 with 6 μg K₃ and 0.8 μg D₃ showed the lowest amount of stimulation (p<0.05). Finally, it can be concluded that 0.4 μg vit D₃ and 6 μg vit K₃ can improve cellular and humoral immune response in broiler chickens. https://ijas.ut.ac.ir/article_59790_3b4506dfe58e56c728f4c72d001bcadd.pdf