پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 47 2 2016 08 22 Identification of novel SNP in promoter of Insulin-Like Growth Factor-I (IGF1) gene in Japanese quail by PCR-SSCP assay Identification of novel SNP in promoter of Insulin-Like Growth Factor-I (IGF1) gene in Japanese quail by PCR-SSCP assay 303 312 59035 10.22059/ijas.2016.59035 FA Homa Aarabi Former Ph.D. Student, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran Mohammad Moradi Shahrbabak Professor, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran 0000-0002-5255-609X Abbas Pakdel Associate Professor, Faculty of Agriculture, University of Isfahan, Iran Hossein Moradi Shahrbabak Assistant Professor, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran Ali Esmailizadeh Koshkoiyeh Professor, Faculty of agriculture, Shahid Bahonar University of Kerman, Iran Journal Article 2015 09 30 Insulin-like growth factor-1 gene (IGF1) is a biological candidate gene for investigation traits such as growth, body weight and muscles growth in different species. In this research eight pairs of white and wild quails were crossed reciprocally as a base population. A total of 34 quails were produced in first generation (F1) and 422 quails were generated by intercrossing the F1 population. Body weights at the time of hatching and different weeks were recorded in the second generation. The association between IGF1 promoter region polymorphism and body weight in different ages was investigated in F2 generation. Using PCR-SSCP assay and sequencing, a novel single nucleotide polymorphism (SNP) was identified in IGF1 promoter region in 472 birds from three Generations. Genotypic frequencies of AA, AG and GG genotypes were 0.05, 0.62 and 0.33, respectively for all generations. The frequency of A and G alleles were 0.36 and 0.64, respectively. The AA genotype was lower than AG and GG genotypes for body weights in ages of one to five weeks and slaughter time and the average of daily gain was also lower from week first to four but these differences weren’t significant. The average of daily gain was higher in females compared to males for AG genotype (P<0.001). Comparison between detected allele in the present study with reported allele by other research groups in chickens revealed a novel SNP in promoter of IGF1 gene in Japanese quail, but association between this polymorphism with body weight and growth rate were not significant. Insulin-like growth factor-1 gene (IGF1) is a biological candidate gene for investigation traits such as growth, body weight and muscles growth in different species. In this research eight pairs of white and wild quails were crossed reciprocally as a base population. A total of 34 quails were produced in first generation (F1) and 422 quails were generated by intercrossing the F1 population. Body weights at the time of hatching and different weeks were recorded in the second generation. The association between IGF1 promoter region polymorphism and body weight in different ages was investigated in F2 generation. Using PCR-SSCP assay and sequencing, a novel single nucleotide polymorphism (SNP) was identified in IGF1 promoter region in 472 birds from three Generations. Genotypic frequencies of AA, AG and GG genotypes were 0.05, 0.62 and 0.33, respectively for all generations. The frequency of A and G alleles were 0.36 and 0.64, respectively. The AA genotype was lower than AG and GG genotypes for body weights in ages of one to five weeks and slaughter time and the average of daily gain was also lower from week first to four but these differences weren’t significant. The average of daily gain was higher in females compared to males for AG genotype (P<0.001). Comparison between detected allele in the present study with reported allele by other research groups in chickens revealed a novel SNP in promoter of IGF1 gene in Japanese quail, but association between this polymorphism with body weight and growth rate were not significant. https://ijas.ut.ac.ir/article_59035_05ec68c53c61ca9a6365d15b67792683.pdf