پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 47 2 2016 08 22 Investigation of polymorphism of DGAT1 gene in Iranian buffaloes Investigation of polymorphism of DGAT1 gene in Iranian buffaloes 175 183 59022 10.22059/ijas.2016.59022 FA Masoumeh Naserkheil Ph.D. Student, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj Seyed Reza Miraie-Ashtiani Professor, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran 0000-0003-3597-4385 Mostafa Sadeghi Associate Professors, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran 0000-0002-7145-622x Ardeshir Nejati-Javaremi Associate Professors, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran 0009-0006-4374-4343 Deukhwan Lee Professor, Department of Animal Biotechnology, Graduate School of Bio. & Technology, Hankyong National University Journal Article 2015 11 20 Diacylglycerol acyltransferase1 (DGAT1) is a key enzyme in synthesis of triglycerides. A transition mutation resulting substitution of guanine by adenine in DGAT1 gene and substitution of lysine by alanine in diacylglycerol-acyltransferase enzyme in cattle has a major effect on milk yield and milk composition traits. In this research, the polymorphism of the region spanning exon 3 to exon 17 of the DGAT1 gene of three indigenous Iranian buffalo populations was investigated. A total number of samples of 200 buffaloes (Khuzestani, Shomali and Azari) were collected to extract DNA and the desired fragments were amplified by polymerase chain reaction (PCR). Genotyping was performed using direct sequencing and analyzed by BioEdit software. Sequence analysis showed novel SNPs in comparative to the reference sequence GenBank (DQ886485) at nucleotide positions g.6097A>G, g.7036C>T, g.7338G>A, g.7710C>T, g.8087C>T, g.8259G>A, g.8275G>A and g.8426C>T of DGAT1 gene in buffalo populations. A comparison with Indian buffalo revealed three exonic SNPs, one of which was non-synonymous. A Unique 22 base insertion has been observed in the intron 10. The results described here genetic diversity and could be useful in genetic and breeding programs in Iranian buffalo. Diacylglycerol acyltransferase1 (DGAT1) is a key enzyme in synthesis of triglycerides. A transition mutation resulting substitution of guanine by adenine in DGAT1 gene and substitution of lysine by alanine in diacylglycerol-acyltransferase enzyme in cattle has a major effect on milk yield and milk composition traits. In this research, the polymorphism of the region spanning exon 3 to exon 17 of the DGAT1 gene of three indigenous Iranian buffalo populations was investigated. A total number of samples of 200 buffaloes (Khuzestani, Shomali and Azari) were collected to extract DNA and the desired fragments were amplified by polymerase chain reaction (PCR). Genotyping was performed using direct sequencing and analyzed by BioEdit software. Sequence analysis showed novel SNPs in comparative to the reference sequence GenBank (DQ886485) at nucleotide positions g.6097A>G, g.7036C>T, g.7338G>A, g.7710C>T, g.8087C>T, g.8259G>A, g.8275G>A and g.8426C>T of DGAT1 gene in buffalo populations. A comparison with Indian buffalo revealed three exonic SNPs, one of which was non-synonymous. A Unique 22 base insertion has been observed in the intron 10. The results described here genetic diversity and could be useful in genetic and breeding programs in Iranian buffalo. https://ijas.ut.ac.ir/article_59022_f6c5d3cfc2a8301e92959f0243eabf65.pdf