پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 42 3 2011 12 22 An Association between the C>T Single Nucleotide Polymorphism of Bovine OPN Gene and Milk Production and Composition in Iran Holstein Cattle An Association between the C>T Single Nucleotide Polymorphism of Bovine OPN Gene and Milk Production and Composition in Iran Holstein Cattle 199 205 23539 FA Majid Pasandideh Mohammadreza Mohammad-abadi Alireza Torang Ali Esmaeilizadeh Kashkoueiyejh Journal Article 1970 01 01 Quantitative trait loci (QTL) affecting milk production traits on bovine chromosome 6 close to the OPN gene location have been identified through several whole genome scans. OPN is a phosphoprotein, synthesized in a variety of tissues and cells and secreted into body fluids. In this study, DNA was isolated from blood samples collected from 398 Holstein cows in Tehran and Esfahan provinces. DNA isolation was performed using salting out method. RFLP-PCR method was used for genotyping of SNP C>T at position 8514 in OPN gene. Frequencies of CC, CT and TT genotypes were estimated as 19, 57 and 24%, respectively. Hardy-Weinberg Equilibrium (HWE) was evaluated using ? square test. The genotypes in this locus deviated from HWE. In this study, significant associations were observed between c.8514C>T genotypes in OPN gene and FATP2X, PROPER305 traits. The SNP, showing signi?cant association with milk traits would provide an excellent opportunity for marker assisted selection programs in dairy cattle. Quantitative trait loci (QTL) affecting milk production traits on bovine chromosome 6 close to the OPN gene location have been identified through several whole genome scans. OPN is a phosphoprotein, synthesized in a variety of tissues and cells and secreted into body fluids. In this study, DNA was isolated from blood samples collected from 398 Holstein cows in Tehran and Esfahan provinces. DNA isolation was performed using salting out method. RFLP-PCR method was used for genotyping of SNP C>T at position 8514 in OPN gene. Frequencies of CC, CT and TT genotypes were estimated as 19, 57 and 24%, respectively. Hardy-Weinberg Equilibrium (HWE) was evaluated using ? square test. The genotypes in this locus deviated from HWE. In this study, significant associations were observed between c.8514C>T genotypes in OPN gene and FATP2X, PROPER305 traits. The SNP, showing signi?cant association with milk traits would provide an excellent opportunity for marker assisted selection programs in dairy cattle. https://ijas.ut.ac.ir/article_23539_f73a80ff3bd9389b0929ae9fa0266a57.pdf