پردیس کشاورزی و منابع طبیعی دانشگاه تهران Iranian Journal of animal Science 2008-4773 56 3 2025 09 23 The effect of vitamin and mineral injection at the start of transition period of Holstein dairy cows on changes in the hematology of cows and the hematology and immune transmission to newborn calves The effect of vitamin and mineral injection at the start of transition period of Holstein dairy cows on changes in the hematology of cows and the hematology and immune transmission to newborn calves 597 615 100528 10.22059/ijas.2024.386110.654043 FA Mohammad Asadi Dept. of Animal Sciences Research, Agricultural and Natural Resources Research and Education Center of Gorgan, Gorgan, Iran. 0000-0001-8199-3953 REZA Kamali Dept. of Animal Sciences Research, Agricultural and Natural Resources Research and Education Center of Gorgan, Gorgan, Iran. 0009-0002-2879-7720 Nader Asadzadeh animal and Poultry Management Research Department, National Institute of Animal Sciences Research, Karaj, Iran. 0000-0002-8176-6918 Journal Article 2024 11 30 The present study was conducted to investigate the effect of mineral and vitamin injection in late pregnancy on hematological changes in cows and hematology and immune transfer to their calves using 32 transition cows in a completely randomized contrasted design with 4 treatments and 8 replications.Experimental treatments included: 1) control (no vitamin and mineral supplement injection), 2) treatment receiving injectable vitamin supplement, 3) treatment receiving injectable mineral supplement and 4) treatment receiving vitamin and mineral supplement injection together. The supplements were injected 28 days before calving. Vitamin supplements included vitamins A, D3, E, B1, B2, B3, B6, B12, C, and mineral supplements included calcium, copper, magnesium, and phosphorus. The injection dose of mineral supplement was 100 ml and the injection dose of vitamin supplement was 20 ml. To examine hematological parameters, blood samples were taken from cows 21 days before calving, on the day of calving, and 21 days after calving. Also, the immunoglobulin status of the calves was measured on birthday, 24 hours after birth, and 30 days after birth, and samples were taken on the 30th day to examine their hematological parameters. The results showed that the injection of minerals, vitamins, and minerals and vitamins increased the concentration of hemoglobin and monocytes in cows before calving and on the day of calving (P<0.05). On the other hand, IgG and IgM were associated with an increase in calves born to mothers receiving supplements (P<0.05). As a result, simultaneous injection of minerals and vitamins can improve the function of the immune system of cows and the immune status and health of calves. The present study was conducted to investigate the effect of mineral and vitamin injection in late pregnancy on hematological changes in cows and hematology and immune transfer to their calves using 32 transition cows in a completely randomized contrasted design with 4 treatments and 8 replications.Experimental treatments included: 1) control (no vitamin and mineral supplement injection), 2) treatment receiving injectable vitamin supplement, 3) treatment receiving injectable mineral supplement and 4) treatment receiving vitamin and mineral supplement injection together. The supplements were injected 28 days before calving. Vitamin supplements included vitamins A, D3, E, B1, B2, B3, B6, B12, C, and mineral supplements included calcium, copper, magnesium, and phosphorus. The injection dose of mineral supplement was 100 ml and the injection dose of vitamin supplement was 20 ml. To examine hematological parameters, blood samples were taken from cows 21 days before calving, on the day of calving, and 21 days after calving. Also, the immunoglobulin status of the calves was measured on birthday, 24 hours after birth, and 30 days after birth, and samples were taken on the 30th day to examine their hematological parameters. The results showed that the injection of minerals, vitamins, and minerals and vitamins increased the concentration of hemoglobin and monocytes in cows before calving and on the day of calving (P<0.05). On the other hand, IgG and IgM were associated with an increase in calves born to mothers receiving supplements (P<0.05). As a result, simultaneous injection of minerals and vitamins can improve the function of the immune system of cows and the immune status and health of calves. https://ijas.ut.ac.ir/article_100528_d4a23f595d61dbaeb97773096e98c817.pdf