ORIGINAL_ARTICLE
The survey of genetic susceptibility to bovine spongiform encephalopathy among Iranian native and Holstein cattle breeds
In recent years, the relationship between insertion and deletion (indel) polymorphisms in promoter region (23 bp) and intron 1 (12 bp) of PRNP gene (Prion protein coding gene) and their relationship to susceptibility of classical bovine spongiform encephalopathy (BSE) have been reported. Insertions of these two polymorphisms increase resistance to classical BSE, while the deletions of these two polymorphisms cause more susceptibility to classical BSE. In this study DNA of Iranian Holstein (n=50), Golpayegani (n=62) and Sistani (n=60) was extracted by modified salting out method. The genes were amplified using specific primers and the genotypes were detected on polyacrylamide gels. Allelic data were tested by Exact Fisher test and genotypic and haplotypic data were tested using Chi-square test. The results showed that considering locus of three mentioned breeds were in Hardy-Weinberg equilibrium. The allelic, genotypic and haplotypic frequencies of the polymorphism of the mentioned genes were estimated in three Iranian cattle breeds and were compared among breeds under this study and the healthy and BSE-affected group of the German cattle (described by Sander et al., 2004). According to the results of this study, if these two regions are the only regions affecting on the classical BSE, Golpayegani cattle have more resistant than healthy and BSE-affected of German cattle.
https://ijas.ut.ac.ir/article_51811_3bf7774e9001a23719a0e31729de92dc.pdf
2014-07-23
89
97
10.22059/ijas.2014.51811
genetic resistance
Golpayegani
mad cow disease
Sistani
Polymorphism
PRNP
Saber
Mohammad Maghsoodi
sm.maghsoodi@gmail.com
1
MSc. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran , Karaj, Iran
AUTHOR
Seyed Reza
Miraei-Ashtiani
ashtiani@ut.ac.ir
2
Professor, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran , Karaj, Iran
LEAD_AUTHOR
Hassan
Mehrabani Yeganeh
hmehrbani@ut.ac.ir
3
Assistance Professor, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran , Karaj, Iran
AUTHOR
Mohammad Hossein
Banabazi
ijhs@can.ut.ac.ir
4
Assistance Professor, Department of Biotechnology, Animal Science Research Institution of Iran, Karaj, Iran
AUTHOR
ORIGINAL_ARTICLE
Comparison the effects of rosemary, thyme, propolis, antibiotic and probiotic on broiler performance
To compare the effects of two herbal extracts, antibiotic, probiotic, and ethanol extract of propolis on performance, relative weight of internal organs, carcass characteristics and morphology of the small intestine of broilers challenged with Salmonella Enteritidis a trial was performed based on completely randomized design. Four hundred and twenty day old male broiler chicks including 7 treatments with 4 replicates of 15 birds per replicate were reared for six weeks. On day 10, except for the negative control group, other groups were inoculated orally with Salmonella Enteritidis. The results showed that antibiotic had significant effect on the relative weight of the breast, increased the relative carcass weight and enlargement of the liver compared to the other groups improving its metabolism and feed conversion ratio. This study showed that plant extracts in addition impact on abdominal fat reduction can increase the height and crypt depth in the small intestine and provide a greater surface area for absorption. In addition, the maximum amount of feed intake and weight gain were observed in the propolis fed group.
https://ijas.ut.ac.ir/article_51812_23d2b1165d481fd6762d44ef58b5c959.pdf
2014-07-23
99
106
10.22059/ijas.2014.51812
herbal extracts
Salmonella Enteritidis
small intestine
Masoud
Taher
masoudtaher64@gmail.com
1
Student, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Iran
AUTHOR
Shaban
Rahimi
rahimi_s@modares.ac.ir
2
Professor, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Iran
LEAD_AUTHOR
Mohammad-Amir
Karimi Torshizi
karimitm@modares.ac.ir
3
Assistant Professor, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Iran
AUTHOR
Abbas
Ashouri
sayeashouri@yahoo.com
4
Student, Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Iran
AUTHOR
ORIGINAL_ARTICLE
Effect of chromium methionine and energy source on production and nutrient digestibility of Holstein cows in prepartum and postpartum
In this study, 32 multiparous non lactating Holstein cows were used to investigate the effects of chromium-l-methionine (Cr-Met) supplementation and dietary energy source on production and nutrient digestibility during the periparturient period. Cows were fed a total mixed ration from 28 days before anticipated calving through 28 days after calving. The study was designed as a randomized complete block design with 2 (energy sources) × 2 (Cr-Met levels) factorial arrangement. Energy sources in the prepartum were fish oil and grain, and were fish oil and palm fat powder in the postpartum. The Cr-Met was supplemented at dosages of 0 or 0.08 mg of Cr/kg of metabolic body weight. Fish oil Supplementation had no significant effect on prepartum dry matter intake (DMI) but tended to decrease postpartum DMI (P< 0.1). Supplemental Cr-Met increased prepartum and postpartum DMI significantly (P<0.05). The effect of treatments on milk production was not significant. No differences were detected in milk composition except for fat percentage in which cows were fed the fish oil had lower percentage of fat compare to others. Supplemental Cr-Met had no significant effect on nutrient digestibility in the prepartum and postpartum. Use of fish oil had no effect on nutrient digestibility in the prepartum, but decreased digestibility of dry matter, organic matter, and crude protein in the postpartum(P<0.05). Results of this study indicate no significant interaction between chromium supplementation and energy source.
https://ijas.ut.ac.ir/article_51813_79bd203965c3238eecf1530ae1344c36.pdf
2014-07-23
107
115
10.22059/ijas.2014.51813
Cattle
chromium
digestibility
fish oil
Milk
Mahdi
Eftekhari
eftekharimahdi@yahoo.com
1
PhD Candidate, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
Aboulfazl
Zali
a.zali@ut.ac.ir
2
Associate Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
LEAD_AUTHOR
Mahdi
Dehghan Banadaki
dehghanb@ut.ac.ir
3
Associate Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
Mahdi
Ganjkhanlou
ganjkhanlou@ut.ac.ir
4
Assistant Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
ORIGINAL_ARTICLE
The effect of methionine on Guinea pig ovulation
The current study intends to analyze the effects of methionine on the ovulation of guinea pigs. 60 guinea pigs were given 4 different treatments in a completely randomize design. The treatments contained 0, 2, 4 and 6 percent of methionine per kg as fed. After two weeks as adaptation period, the four different treatments were given to the animals for two estrus cycle. After synchronization which was achieved through the inter muscular injection of PGF2a analog (cloroprostonal). 6ml/kg of cloroprostonal was injected twice in an eleven-day interval. At the end of experiment guinea pigs were slaughtered and the numbers of mature follicles were calculated by loop microscopy. The result shown that, the number of mature follicle increased as the amount of methionine in the diet rased. The Duncan`s test showed a significant relationship between the number of follicle in the right and left ovary with the higher amount of methionine (P<0.05) and didn’t reflect a significant relationship between weight of two ovary and the amount of methionine. It seems that if the amount of methionine increases in the diet, the ovulation will also increase regardless of the ovary and body weight.
https://ijas.ut.ac.ir/article_51814_43b005df8f8f43ec116c9fba5e371d70.pdf
2014-07-23
117
124
10.22059/ijas.2014.51814
methionine
guinea pig
ovulation
Rasoul
Karimi
karimi_rasoul62@yahoo.com
1
دانشجوی کارشناسی ارشد فیزیولوژی دام دانشگاه تهران
LEAD_AUTHOR
Malek
Shakeri
mshaker@ut.ac.ir
2
استادیار بخش فیزیولوژی دام دانشگاه تهران
AUTHOR
Mahdi
Zhandi
mzhandi@ut.ac.ir
3
استادیار بخش فیزیولوژی دام دانشگاه تهران
AUTHOR
ORIGINAL_ARTICLE
Determining the effect of estradiol, oxytocin, prostaglandin E1 and relaxin on cervix dilation and pregnancy rate of Zandi breed sheep
The aim of this study was to evaluate the effect of estradiol (E2), oxytocin (OT), relaxin (relaximol) injection and prostaglandin E1 (misoprostol) suppository on the cervix dilation, time of cervical dilation persistency and pregnancy rate of Zandi ewes using artificial insemination. Eighty 3-4 years old and 55±2.5 kg weight ewes were used for this study. In experiment 1, the ewes were assigned to four equally groups. The groups were received 100 µl E2, 100 IU of OT, 5 ml relaximol and 200 µg misoprostol, respectively. Then, cervical dilation and time of cervical dilation persistency were examined at four times. E2 had no effect on cervical dilation and time of cervical dilation persistency (P>0.05). Using of OT, relaximol and misoprostol lead to cervical dilation (P<0.05). In experiment 2, the estrous synchronization was accomplished using CIDR insertion for 12 days. At the time of CIDR removal, 500 IU of eCG injected in to the ewes and then assigned to four equally groups. The control group received no cervical dilator treatment. Other three groups received the same treatment as the first experiment. According to the best time of cervical dilation in experiment 1, ewes were inseminated transcervically in the times of 20 and 40 min and 5 hours after treating, respectively, at 54 hours after eCG injection. Fifty days after insemination, pregnancy rate were recognized via ultrasonography diagnosis. Pregnancy rate in OT group was higher (P<0.05) than misoprostol and relaximol groups (60% vs 25 and 10%, respectively). However, there were no significant differences (P>0.05) between oxytocin and control group (60% vs 50%) in pregnancy rate. In conclusion, injection of oxytocin dilate the cervical canal and improved pregnancy rate in Zandi ewes, therefore this could be used as an instrument for improving pregnancy rate during artificial insemination.
https://ijas.ut.ac.ir/article_51815_8e62a3355aa3a029258f3c8a5c14ce08.pdf
2014-07-23
125
133
10.22059/ijas.2014.51815
cervix
estradiol
misoprostol
oxytocin
pregnancy rate
relaximol
Reza
Masoudi
masoomi.reza@gmail.com
1
PhD., University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
Ahmad
Zare-Shahneh
azareh@ut.ac.ir
2
Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
LEAD_AUTHOR
Armin
Tohidi
atowhidi@ut.ac.ir
3
Associate Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
Hamid
Kahram
hamid_kohram@yahoo.com
4
Assistance Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
Abbas
Akbari Sharif
sharif.8784@yahoo.com
5
MSc
AUTHOR
Mohsen
Sharafi
sharafi2000@gmail.com
6
Assistance Professor, University College of Agriculture & Natural Resources, Tarbiyat Modares University, Iran
AUTHOR
Vahid
Zahedi
vahid.zahedi@yahoo.com
7
MSc. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
ORIGINAL_ARTICLE
The effect of incomplete sire pedigree on prediction of breeding value and estimation of the genetic trend of weaning weight and average daily gain in sheep
In order to study on the effect of incomplete sire's pedigree on the prediction of breeding value and estimation of genetic trend, two quantitative traits such as weaning weight and average daily gain with heritabilities 0.15 and 0.30 were simulated. Phenotypic, genetic and environmental correlations were assumed 0.5. Resulted data files with different proportion of missed sire pedigree (0-100) were analyzed using bivariate animal model. Breeding value means for WW and ADG reduced from 1.61 to 0.55 and 69.48 to 24.68 with 10 percent random deletion of sire pedigree. This parameter was also decreased to 1.47 and 63.67 for WW and ADG respectively, with 10 percent sequential deletion of sire pedigree. Decreasing genetic trend of ADG were 61.32 and 0.66 percent for 10 percent random and sequential deletion of sire pedigree, respectively. This reduction for WW was also 63.43 and 0 percent. Generally, the incompleteness of sire pedigree affected the accuracy of breeding value prediction and genetic trend and based on the rate of sire pedigree deletion, the part of accuracy of breeding value prediction were lost. Then, the genetic tend estimated using real data should be analyzed based on the rate of completeness of sire pedigree.
https://ijas.ut.ac.ir/article_51816_7e0e66bbcf4292b4f0f021fbf6f30863.pdf
2014-07-23
135
141
10.22059/ijas.2014.51816
computer simulation
genetic gain
missed sire pedigree
Mokhtar-Ali
Abbasi
pmaz_abbasi@yahoo.com
1
Associated Professor, Animal Science Research Institute, Karaj, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Determining ofnutritive value of Cressa Cretica plant in different phenological of growth stageswith in vitro, in vivo and nylon bag methods in Sistan
The aim of this research was determination of nutritive value of Cressa Cretica with gas test method, in vivo digestibility and degradability with nylon bag method in three phenological stages. The amount of crude protein decreased significantly in seeding stage. But the amount of NDF and ADF increased in seeding stage. The degradability of dry matter and rapid and slowly degradable fractions decreased in development of phenological stages. The gas production increased in seeding stage compared to vegetative and flowering stage. The amount of organic matter digestibility for three phenologic stages was %56.34, %58.04 and %58.44 respectively and metabolizable energy was %8.4, %8.71 and %8.69 MJ/kg respectively (P<0.01). The apparent digestibility of NDF and ADF of this plant decreased numerically in sheep with increasing of lignifications of cell wall in flowering and seeding stage. The result of this experiment showed significant effect of different stages of growth on nutritive value of Cressa cretica. With regards of results of chemical composition and apparent digestibility vegetative growth of Cressa cretica had higher nutritive value compared to flowering and seeding stage. Nevertheless in addition survey of antinutrient of this plant, economy stage of harvest and effect of different use on nutritive value of this plant is determined in future researches.
https://ijas.ut.ac.ir/article_51817_d909f53f67ac953ff706179cb645eb5e.pdf
2014-07-23
143
152
10.22059/ijas.2014.51817
Cressa Cretica
Degradability
digestibility
Gas production
Phenological stages
Taghi
Najafi
taghinajafi17@yahoo.com
1
niversity of zabol
AUTHOR
Mohammad-reza
Dehghani
mohrezadehghani@yahoo.com
2
دانشیار، دانشگاه زابل
LEAD_AUTHOR
Mostafa
Yousef-elahi
m_yousefelahi@uoz.ac.ir
3
university of zabol
AUTHOR
ORIGINAL_ARTICLE
Effect of administration of olive oil in yolk- sac on growth performance and breast development in broiler chickens exposed to post- hatch fasting stress
This experiment was carried out to investigate the effects of in yolk-sac administration of olive oil (0 and 0.7 ml) on productive performance and breast development of broiler chickens exposed to post-hatch fasting stress (0, 12, 24, 36 and 48 hours). A total number of 800, 1-day-old broiler chicks in a completely randomized block design with 10 treatments and 4 replicates were used. Live body weight of broiler chicks exposed to 24 and 36 hours post-hatch fasting was significantly lower compared to control group (P<0.05). Extending of post hatch fasting up to 48 hours induced compensatory growth in broiler. Administration of olive oil to yolk sac significantly increased mortality rate and negatively affected production efficiency index (P<0.05). In day 28, fasting and administration of olive oil showed no significant effect on breast external dimensions and on major and minor pectoral is muscles weight (P<0.05). It is concluded that 24 and 36 hours Post-hatch fasting had negative effect on broiler performance and that injection of 0.7 ml olive oil was reduced performance of broiler chickens exposed to post-hatch fasting.
https://ijas.ut.ac.ir/article_51818_59892883a629143a4c22c7c3dc25d523.pdf
2014-07-23
153
161
10.22059/ijas.2014.51818
broiler chick
early nutrition
Olive oil
post-hatch stress
Zahra
Khorrami
zahrakhorrami@rocketmail.com
1
MSc. Student, Department of Animal Sciences, Lorestan University, Iran
AUTHOR
Heshmatollah
Khosravinia
khosravi_fafa@yahoo.com
2
Associate Professor, Department of Animal Sciences, Lorestan University, Iran
LEAD_AUTHOR
Arash
Azarfar
arash.azarfar@gmail.com
3
Associate Professor, Department of Animal Sciences, Lorestan University, Iran
AUTHOR
Akbar
Cheraghi
akbar.cheraghi00@gmail.com
4
MSc., Department of Animal Sciences, Lorestan University, Iran
AUTHOR
ORIGINAL_ARTICLE
Effects of inclusion of dried deoiled Satureja khuzistanica in finishing diet of lambs on kinetics of gas production in vitro
The current experiment was carried out to investigate the possible effects of replacing alfalfa by dried de-oiled Savory (Satureja khuzistanica; DDS) at the levels of 0, 25, 50, 75 and 100 percent in finishing diets of Mehraban lambs on kinetics of gas production in vitro. The experimental diets contained 20% alfalfa and 80% of concentrate on dry matter basis. Replacing alfalfa by DDS had no significant effect on gas production at different incubation times as well as on kinetics of gas production including asymptotic of gas production (A), fraction rate of gas production (C) and half time of gas production (t1/2) (P>0.05). Concentration of ammonia was significantly decreased when alfalfa was replaced by DDS in the diets (P<0.05). Experimental diets did not have significant effect on total volatile fatty acids. Although apparent in vitro dry matter digestibility was significantly decreased (P<0.05) when alfalfa was replaced by dried DDS at the levels of 75 and 100%, true dry matter digestibility remained unaffected. Replacing alfalfa by DDS up to 75% tended to increase microbial biomass production (MBP) and efficiency of MBP. The results of current study showed that inclusion of DDS in finishing diet of lambs could reduce ammonia production; thereby improve nitrogen utilization without having any adverse effect on digestibility of dry matter.
https://ijas.ut.ac.ir/article_51819_341f06d1b0bdc16a046634ab625cddc4.pdf
2014-07-23
163
171
10.22059/ijas.2014.51819
finishing lambs
dried deoiled Satureja khuzistanica
kinetics of gas production
Seyede Soheila
Noshadi
nowshadi7star@gmail.com
1
MSc. Student, Department of Animal Sciences, University of Lorestan, Iran
AUTHOR
Arash
Azarfar
arash.azarfar@gmail.com
2
Associate Professor, Department of Animal Science, University of Lorestan, Iran
LEAD_AUTHOR
Daryoush
Alipour
alipourd@basu.ac.ir
3
Associate Professor, Department of Animal Science, Bu-Ali Sina University, Hamedan, Iran
AUTHOR
Heshmatollah
Khosravinia
khosravi_fafa@yahoo.com
4
Associate Professor, Department of Animal Science, University of Lorestan, Iran
AUTHOR
ORIGINAL_ARTICLE
Lactation curve modeling for milk production traits in Mahabadi goat breed
In this research, in order to describe milk production traits lactation Mahabadi goat breed, seven statistical functions were fitted using 1665 milk production traits records and 833 body weight records. The milk production traits were milk yield, fat percent, fat yield, protein percent, protein yield, lactose percent and SNF percent. These records have been recorded on 123 goats during years of 2012-2013. Based on results, there was not considerable difference in curve fitting of milk production and its composition yield among used functions. However, Ali and Schaeffer and Narushin-Takma functions in fat and SNF percentage, and inverse polynomial and Gompertz functions in protein and lactose percentage had higher goodness of fit in comparison with other function. Average daily milk production, days to peak production, milk production in peak, lactation period, and milk persistency were estimated 1 kg, 22 days, 1.22 kg, 120 days, and 5.59, respectively.
https://ijas.ut.ac.ir/article_51820_4806ca25ba2a7821ec21f9077be4ae49.pdf
2014-07-23
173
184
10.22059/ijas.2014.51820
fat- protein
lactation functions
Mahabadi goat
milk persistency
Abdollah
Rezagholivand Lahroud
rgholivand@gmail.com
1
MSc. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
Mohammad
Moradi Shahrebabak
moradim@ut.ac.ir
2
Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
LEAD_AUTHOR
Abbas
Pakdel
pakdel@cc.iut.ac.ir
3
Associated Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
AUTHOR